Survivin基因在大肠癌中的表达及与端粒酶活性的关系

目的：探讨survivin基因在大肠癌中的表达及与端粒酶活性的关系。方法：采用逆转录聚合酶链反应(RT—PCR)和端区重复扩增法酶联免疫吸附测定(TRAP—ELISA)方法检测大肠癌及其癌旁组织survivin mR—NA表达和端粒酶活性情况。结果：(1)65％大肠癌组织表达survivin mRNA，而癌旁组织25％表达。Survivin基因表达与肿瘤大小、侵袭深度、淋巴结转移及分化程度无明显关系。(2)大肠癌组织端粒酶活性阳性率为90％，明显高于癌旁组织的5％，端粒酶表达阳性与大肠癌淋巴结转移、分化不良程度显著相关。(3)Survivin mRNA表达与端粒酶活性明显相关。结论：Survivin基因在大肠癌发生发展中可能起作用。端粒酶可作为诊断大肠癌的标志物。Survivin表达上调和端粒酶激活可能通过各自不同的信号途径在大肠癌变中发挥协同作用。

Survivin Gene Expression and its Correlation with Telomerase Activity in Colorectal Cancer

Objective:To investigate the expression of survivin and its correlation with telomerase activity in colorectal cancer (CRC). Methods: The survivin mRNA expression was detected by RT-PCR, and telomerase activity was measured by TRAP-ELISA method in 20 specimens of human colorectal cancer and adjacent tissues. Results:(1)Expression of survivin mRNA was detected in a significantly greater proportion of CRC (65%) than adjacent cancer tissue (25%).There was no relationship between survivin gene expression and tumor size, invasive depth, lympho node metastasis, and differentiation of malignance.(2) The positive rate of telomerase activity was 90%, and was significantly higher than that in adjacent cancer tissue (5%).A significant positive correlation of telomerase activity level with differentiation of malignance and lympho node metastasis was observed(3) The expression of survivin mRNA was correlated with telomerase activity.Conclusion: The up-regulation expression of survivin gene in CRC indicates that survivin may play a role in the pathway of carcinogenesis. Telomerase activation can be used as a diagnostic marker for CRC. Both survivin and telomerase activities may have a strong cooperative effect in the carcinogenesis.