nm23-H1、c-erbB-2基因表达与结直肠癌转移的相关性

目的：探讨ｎｍ２３－Ｈ１、ｃ－ｅｒｂＢ－２基因表达与结直肠癌转移的关系。方法：采用免疫组化ＳＡＢＣ法，检测了８５例结直肠癌中ｎｍ２３－Ｈ１、ｃ－ｅｒｂＢ－２基因的蛋白产物。结果：ＤｕｋｅｓＤ期结直肠癌中ｎｍ２３－Ｈ１基因表达显著低于ＤｕｋｅｓＡ、Ｂ、Ｃ各期（Ｐ＜０．０１），ｃ－ｅｒｂＢ－２基因表达则显著高于ＤｕｋｅｓＡ、Ｂ、Ｃ各期（Ｐ＜０．０１），两基因在ＤｕｋｅｓＡ、Ｂ、Ｃ各期间的阳性表达差异无显著性（Ｐ＞０．０５）。结论：ｎｍ２３－Ｈ１与结直肠癌的肝转移呈负相关，ｃ－ｅｒｂＢ－２呈正相关，但两基因与淋巴结转移均无显著相关；两基因在蛋白水平上存在负相关性

Relationship between expression of nm23 H1, c erbB 2 proteins and metastasis in colorectal carcinoma

Purpose To research the relationship between expression of nm23 H1, c erbB 2 proteins and metastasis in colorectal carcinomas. Methods Immunohistochemical technique was used to detect the protein expression of nm23 H1 and c erbB 2 genes in colorectal carcinoma of different Dukes stages. Results The protein expression of the two genes had no significant difference in the Dukes A, B, C stages (both P >0 05), while in Dukes D stage, protein expression of nm23 H1 was significantly reduced and protein expression of c erbB 2 gene was significantly increased compared to the Dukes A, B, C stages (both P <0 01). Conclusion Protein expression of nm23 H1 gene in colorectal carcinoma is inversely associated with liver metastasis, c erbB 2 gene is positively correlated with liver metastasis. But there was no relationship between expression of the two genes and lymph node metastasis. On protein levels, a reverse relationship can be seen between the two genes.