磁标记大鼠间充质干细胞肝脏移植的磁共振成像活体示踪

目的探讨1.5 T MRI活体示踪磁标记干细胞经门静脉肝脏移植的可行性。方法大鼠间充质干细胞以菲立磁和DAPI标记后,经门静脉注入大鼠肝脏,分别于移植前及移植后1 h、3 d、7 d和14 d行MRI扫描,并与组织荧光显像和铁染色对照。结果移植后1 h、3 d、7 d及14 d肝脏信噪比分别为1.10±0.26、8.18±1.55、11.08±1.30、14.15±1.02,7 d以内肝脏信噪比与移植前比较均有明显降低(P<0.05)。各时相肝组织铁染色与荧光显像的空间分布一致。结论1.5 T MRI活体示踪经门静脉移植的磁标记干细胞是可行的,MRI示踪时间可持续7 d。

Tracing magnetically labeled mesenchymal stem cells transplanted into rat livers by MRI

OBJECTIVE: To trace magnetically labeled MSCs transplanted into the rat livers by magnetic resonance imaging (MRI). METHODS: Feridex and DAPI labeled rat mesenchymal stem cells (MSCs) were injected via portal veins into carbon tetrachloride treated rats. MRI was performed with a clinical 1.5 T MRI machine immediately before the MSCs injection and at h 1, d 3, d 7, and d 14 after the injection, and then the signal-to-noise ratio (SNR) was measured. MRI findings were compared with the liver histopathologies after the slides were stained with fluorescence dye and Prussian blue. RESULTS: The SNR for liver was 1.10+/-0.26 at hour 1, 8.18+/-1.55 at day 3, 11.08+/-1.30 at day 7, and 14.15+/-1.02 at day 14 respectively. Within 7 days after the MSCs transplantation, the SNRs of the livers were significantly lower than those before the transplantation (P less than 0.05). Histologically, the blue fluorescent particles under the fluorescence microscopy matched in distribution with the iron particles on the Prussian blue stained slides. CONCLUSION: The magnetically labeled MSCs transplanted into livers give rise to an obvious signal decrease, and can be tracked with a 1.5 T clinical MRI machine for up to 7 days after MSCs transplantation.