| 热休克蛋白70-HBcAg(18～27)蛋白复合物对乙型肝炎病毒转基因小鼠的免疫研究 |
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| 引用本文： | 胡怀东,胡鹏,陈国林,彭明利,陈敏,任红. 热休克蛋白70-HBcAg(18～27)蛋白复合物对乙型肝炎病毒转基因小鼠的免疫研究[J]. 中华肝脏病杂志, 2007, 15(1): 28-31 |
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| 作者姓名： | 胡怀东 胡鹏 陈国林 彭明利 陈敏 任红 |
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| 作者单位： | 1. 400010,重庆医科大学病毒性肝炎研究所、省部共建感染性疾病分子生物学教育部重点实验室
2. 哈尔滨医科大学第一临床医学院感染病科 |
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| 基金项目： | 国家自然科学基金(30300297) |
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| 摘 要： | 目的研究结核杆菌热休克蛋白(HSP)70-HBcAg(18～27))蛋白复合物对HBV转基因小鼠免疫应答的影响。方法体外构建HSP70-HBcAg(18～27)蛋白复合物,以HBV转基因小鼠为实验对象,进行体内免疫学功能研究。结果HSP70-HBcAg(18～27)蛋白复合物使HBV转基因小鼠外周血及脾中CD4~ T淋巴细胞和CD8~ T淋巴细胞大量增殖,诱导较强的抗原特异性CTL应答,并活化自然杀伤细胞、树突状细胞参与应答反应。结论HSP70-HBcAg(18～27)蛋白复合物具有免疫原性,能够增强转基因鼠细胞免疫应答能力,有望作为蛋白疫苗用于慢性乙型肝炎的免疫治疗。
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| 关 键 词： | 热休克蛋白 肝炎核心抗原 乙型 蛋白质疫苗 细胞毒性T淋巴细胞 小鼠 转基因 |
| 修稿时间： | 2006-06-28 |
Immune response to HSP70-HBcAg(18-27) complex in HBV transgenic mice |
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| HU Huai-dong,HU Peng,CHEN Guo-lin,PENG Ming-li,CHEN Min,REN Hong. Immune response to HSP70-HBcAg(18-27) complex in HBV transgenic mice[J]. Chinese journal of hepatology, 2007, 15(1): 28-31 |
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| Authors: | HU Huai-dong HU Peng CHEN Guo-lin PENG Ming-li CHEN Min REN Hong |
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| Affiliation: | Key Laboratory of Molecular Biology for Infectious Diseases, PRC Ministry of Education, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China |
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| Abstract: | Objective To study the cellular immune response to HSP70-HBcAg_(18-27) complex in HBV transgenic mice.Method HSP70-HBcAg_(18-27) complex was reconstituted in vitro,then it was injected into HBV transgenic mice to observe the cellular immune response.At the same time,we investigated whether HSP70-HBcAg_(18-27) complex could generate antigen specific cytotoxic T lymphocyte responses in spleen cells. Results Our results demonstrated that HSP70-HBcAg_(18-27) complex increased levels of CD4~ and CD8~ T cells in the spleens and peripheral blood of HBV transgenic mice,and the complex also activated dendritic and natural killer cells.Conclusion HSP70-HBcAg_(18-27) complex has an immunological antigenicity in raising the immunoresponse to chronic HBV infection in HBV transgenic mice.HSP70-HBcAg_(18-27) complex might be considered as a can- didate for further studies on its role as a therapeutic vaccine against chronic HBV infection in humans. |
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| Keywords: | Heat shock protein Hepatitis B core antigen Protein vaccine Cytotoxic T lymphocytes Mice transgenic |
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