| 升结肠支撑管腹膜炎动物模型的建立 |
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| 引用本文： | 杨方亮,王新波,王思珍,丁威威,范欣鑫. 升结肠支撑管腹膜炎动物模型的建立[J]. 肠外与肠内营养, 2010, 17(3) |
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| 作者姓名： | 杨方亮 王新波 王思珍 丁威威 范欣鑫 |
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| 作者单位： | 南京大学医学院临床学院(南京军区南京总医院)解放军普通外科研究所江,江苏南京,210002 |
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| 摘 要： | 目的:建立弥漫性腹膜炎动物模型,并观察不同程度弥漫性腹膜炎对肠黏膜通透性的影响。方法:采用升结肠支撑管腹膜炎模型(CASP)诱发弥漫性腹膜炎。将小鼠随机分为对照组、假手术组、和支撑管1.3mm组和1.7mm组。观察术后72h各组的生存率。并于术后18h比较肠黏膜通透性的变化。结果:升结肠支撑管内径越大,造成弥漫性腹膜炎小鼠死亡率越高。1.3mm组和1.7mm组术后72h累积生存率分别为75%和44%。1.7mm组肠道通透性高于1.3mm组。结论:CASP模型较好地模拟了急性弥漫性腹膜炎。当支撑管内径为1.7mm时,可模拟重度急性弥漫性腹膜炎,而当支撑管内径为1.3mm时则可模拟轻度急性弥漫性腹膜炎。
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| 关 键 词： | 升结肠支撑管腹膜炎模型 肠黏膜屏障 肠黏膜通透性 |
The changes of intestinal permeability in the mouse model of colon ascendens stent peritonitis |
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| YANG Fang-liang,WANG Xin-bo,WANG Si-zhen,DING Wei-wei,FAN Xin-xin. The changes of intestinal permeability in the mouse model of colon ascendens stent peritonitis[J]. Parenteral & Enteral Nutrition, 2010, 17(3) |
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| Authors: | YANG Fang-liang WANG Xin-bo WANG Si-zhen DING Wei-wei FAN Xin-xin |
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| Affiliation: | YANG Fang-liang,WANG Xin-bo,WANG Si-zhen,DING Wei-wei,Fan Xin-xin(Research Institute of General Surgery,Clinical School of Medical College of Nanjing University/Nanjing General Hospital of Nanjing military Command,PLA,Nanjing 210002,Jiangsu,China) |
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| Abstract: | Objective:To establish a mouse model of diffuse peritonitis and to investigate the changes of the intestinal permeability in mouse with diffuse peritonitis of varying severity.Methods:In the model of colon ascendens stent peritonitis,2 groups of CASP were established depending on the stent diameter ( 1.3mm group and 1.7mm group).Survival rate was analyzed in the groups.The intestinal permeability was measured by detecting the mucosal-to-serosal clearance of FD4.Results:Survival rate after CASP procedure cor... |
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| Keywords: | Colon ascendens stent peritonitis Gut barrier Intestinal permeability |
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