| 结核杆菌embB基因突变与临床治疗的关系 |
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| 引用本文： | 李洪敏,吴雪琼,王巍,梁建琴,张滔,李燕峰.结核杆菌embB基因突变与临床治疗的关系[J].军医进修学院学报,2004,25(3):219-221. |
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| 作者姓名： | 李洪敏 吴雪琼 王巍 梁建琴 张滔 李燕峰 |
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| 作者单位： | 解放军总医院,第309临床部结核中心实验室,北京,100091 |
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| 基金项目： | ”十五”军队科研基金项目 (0 1MA2 17) |
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| 摘 要： | 目的：了解结核菌耐乙胺丁醇药敏实验和embB基因突变情况，研究其临床应用价值。方法：通过聚合酶链反应(PCR)．单链构象多态性(SSCP)技术初步鉴定96株分支杆菌临床分离株的菌种，并进一步分析其embB基因。结果：分析56株分支杆菌临床分离株的16S rDNA SSCP电泳图谱均与结核分支杆菌标准株相同。21株药物敏感株的embB基因SSCP均泳动正常；35株耐乙胺丁醇分离株中，17侏(48．6％)embB基因SSCP泳动异常。结论：部分结核分支杆菌耐乙胺丁醇是由于其embB基因突变所致。且embB基因突变均发生在药敏实验高浓度区。
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| 关 键 词： | 结核杆菌 基因突变 治疗 药敏实验 embB基因 乙胺丁醇 抗药性 |
| 文章编号： | 1005-1139(2004)03-0219-03 |
| 修稿时间： | 2003年10月21 |
The relationship of the mutation on embB genes and drug-resistence with clinical treatment |
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| LI Hong-min,WU Xue-qiong,WANG Wei,LIANG Jian-qing,ZHANG Tao,LI Yan-feng.The relationship of the mutation on embB genes and drug-resistence with clinical treatment[J].Academic Journal of Pla Postgraduate Medical School,2004,25(3):219-221. |
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| Authors: | LI Hong-min WU Xue-qiong WANG Wei LIANG Jian-qing ZHANG Tao LI Yan-feng |
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| Abstract: | Objective:To realize the relevance of the mutations of embB genes with drug resistence in M. tuberculosis isolates,and to evaluate their clinical value.Methods:96 clinical isolates were identified their mycobacterial species,and then analyzed their embB genes with PCR SSCP. Results:56 clinical isolates all had the same SSCP profiles as M.tuberculosis. 21 drug-sensitive isolates had normal embB SSCP profiles. Of 35 ethambutol-resistant isolates,17(48.6%) displayed abnormal embB SSCP profiles.Conclusions:Ethabutol resistancesin some M.tuberculosis isolates were due to mutations on embB genes,Moreover,the mutation of embB genes has high accidence in isolates which resistanthigh concentration ethambutol. |
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| Keywords: | mycobacterium mycobacteriumtuberculosis drug resistance ethambutol |
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