| 反相高效液相色谱法测定普卢利沙星中的有关物质 |
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| 引用本文： | 刘利军. 反相高效液相色谱法测定普卢利沙星中的有关物质[J]. 中南药学, 2010, 8(6): 423-425 |
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| 作者姓名： | 刘利军 |
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| 作者单位： | 湖南省药品检验所,长沙,410001 |
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| 摘 要： | 目的建立反相高效液相色谱法测定普卢利沙星中的有关物质。方法采用VP-ODS C18（4.6 mm×150mm,5μm）色谱柱,以乙腈-稀磷酸（1→1 000）-1 mol·L^-1戊烷磺酸钠溶液（27∶72∶1）为流动相,流速1.5mL·min^-1,检测波长275 nm,柱温25℃。结果在本文色谱条件下普卢利沙星与相邻杂质峰的分离度〉1.5,普卢利沙星、中间体（A、B、C）和杂质A均能完全分离。结论该方法简便、准确、可行,可用于普卢利沙星的质量控制。
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| 关 键 词： | 反相高效液相色谱法 普卢利沙星 有关物质 |
Determination of relevant substances in prulifloxacin by RP-HPLC |
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| LIU Li-jun. Determination of relevant substances in prulifloxacin by RP-HPLC[J]. Central South Pharmacy, 2010, 8(6): 423-425 |
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| Authors: | LIU Li-jun |
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| Affiliation: | LIU Li-jun(Hunan Institute of Drug Control,Changsha 410001) |
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| Abstract: | Objective To establish a reversed phase high performance lipuid chromatography method for the determination of relevant substances in prulifloxacin.Methods VP-ODS C18 column（4.6 mm×150 mm,5 μm） was used,the mobile phase consisted of acetonitrile-dilute phosphoric acid（1→1 000）-1 mol·L^-1 n-pentane sulfonate solution（27∶72∶1） at 1.5 mL·min^-1,and the column temperature was 25 ℃.Results The separating size of prulifloxacin and its adjacent impurity chromatographic peak was more than 1.5.Prulifloxacin and intermediate（A,B,C） and impurity A were seperated well.Conclusion The method is simple,accurate and reproducible.It can be used to control the quality of prulifloxacin. |
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| Keywords: | RP-HPLC prulifloxacin relevant substance |
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