| 3,5-二硝基水杨酸法测定蔓菁中还原糖和总糖含量 |
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| 引用本文： | 高文军,李卫红,王喜明,乔丽芳. 3,5-二硝基水杨酸法测定蔓菁中还原糖和总糖含量[J]. 中国药业, 2020, 0(9): 113-116 |
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| 作者姓名： | 高文军 李卫红 王喜明 乔丽芳 |
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| 作者单位： | 山西省医药与生命科学研究院 |
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| 基金项目： | 山西省科技厅面上自然基金项目[201801D121296]。 |
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| 摘 要： | 目的建立测定晋南蔓菁还原糖和总糖含量的3,5-二硝基水杨酸比色(DNS)法。方法以葡萄糖为对照品,DNS试剂的用量为2.0 mL,90℃水浴加热6 min,于540 nm波长下测定蔓菁中还原糖和总糖含量。结果还原糖和总糖平均加样回收率分别为101.66%和96.44%,RSD分别为2.86%和1.71%(n=6);3批蔓菁中还原糖和总糖的含量分别为5.86%~6.93%和44.65%~46.50%。结论该方法简便、准确、重复性好,可用于检测蔓菁中还原糖和总糖的含量。
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| 关 键 词： | 蔓菁 还原糖 总糖 3,5-二硝基水杨酸 含量测定 |
Determination of Reducing Sugar and Total Sugar in Turnip by 3,5-Dinitrosalicylic Acid Colorimetry |
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| GAO Wenjun,LI Weihong,WANG Ximing,QIAO Lifang. Determination of Reducing Sugar and Total Sugar in Turnip by 3,5-Dinitrosalicylic Acid Colorimetry[J]. China Pharmaceuticals, 2020, 0(9): 113-116 |
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| Authors: | GAO Wenjun LI Weihong WANG Ximing QIAO Lifang |
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| Affiliation: | (Shanxi Institute of Medicine and Life Sciences,Taiyuan,Shanxi,China 030006) |
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| Abstract: | Objective To establish a 3,5-dinitrosalicylic acid colorimetry(DNS)method for the determination of reducing sugar and total sugar content in Jinnan turnip.Methods Glucose was used as the reference substance;the amount of DNS reagent was 2.0 mL,heated in a 90°C water bath for 6 min,and measured at 540 nm to determine the content of reducing sugar and total sugar in Jinnan turnip.Results The average recoveries of reducing sugar and total sugar were 101.66%and 96.44%,respectively;the RSDs were 2.86%and 1.71%,respectively(n=6).The content of reducing sugar and total sugar in 3 batches of turnip were 5.86%-6.93%,44.65%-46.50%,respectively.Conclusion The method is simple,accurate,and reproducible,and can be used to detect the reducing sugar and total sugar content in turnip. |
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| Keywords: | turnip reducing sugar total sugar 3 5-dinitrosalicylic acid content determination |
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