针刀疗法对肩周炎模型兔肩关节囊局部组织中基因蛋白表达的影响

目的:研究针刀疗法对肩周炎兔肩关节囊中白细胞介素6(IL-6)、高迁移率族蛋白1(HLGB1)、酸敏感离子通道蛋白3(ASIC3)蛋白和基因表达的影响.方法:健康雄性新西兰大白兔30只,按照随机数字表法分为空白组、模型组、针刀组,每组各10只.采用机械加冰敷制造肩周炎模型.密切关注造模前后各组肢体功能活动变化;采用HE...

Effects of acupotomy on the expression of genes and proteins in a rabbit model of scapulohumeral periarthritis

Objective:To study the effects of acupotomology on the expression of IL-6,HLGB1,ASIC3 and genes in periarthritis humeral rabbits.Methods:30 healthy male New Zealand white rabbits.Divided into blank,model,and acupotomy groups according to the random number table method,with 10 pieces in each group.Fabrication of a frozen shoulder model using mechanical plus ice application.Pay close attention to the changes in functional activity of each group of limbs before and after modeling,local histomorphological changes were observed using HE staining,detection of local IL-6,HLGB1 and ASIC3 protein levels by Western blot method.The mRNA expression levels of IL-6,HLGB1 and ASIC3 in synovium of joint capsule were detected by RT-PCR.Results:Acupotomy release improves localized pathology of the shoulder capsule,reduces synovial and interstitial fibrillar effusion,adhesions and mechanization and promotes tissue repair.Significantly increased IL-6,HLGB1,ASIC3 gene and protein expression levels in the rabbit shoulder synovium in the model group compared with the blank group(all P<0.05).The expression levels of IL-6,HLGB1 and ASIC3 genes and proteins were significantly reduced in the rabbit shoulder synovium in the acupotomy group compared with the model group(all P<0.05).The content of IL-6,HLGB1,and ASIC3 proteins in the model group was significantly increased and the gene expression level was significantly up-regulated,which was significantly different from the blank group(all P<0.05),significantly lower protein content of the above indicators in the acupotomy group,and has a significant inhibitory effect on the expression level of these genes,significantly different compared to the model and blank group(all P<0.05).Conclusion:Acupotomy therapy reduces IL-6,HLGB1,ASIC3 protein expression levels in the shoulder bursa synovium,this may be one of the mechanisms by which acupuncture works effectively in treating scapulohumeral periarthritis.