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| 胰升糖素样肽1对乳鼠心肌细胞缺氧/复氧损伤的保护机制 | |
| 引用本文： | 谢云,沙伟伟,周雪,王绍欣,汪玮琳,韩丽萍,王家驰,于德民. 胰升糖素样肽1对乳鼠心肌细胞缺氧/复氧损伤的保护机制[J]. 中华内分泌代谢杂志, 2010, 26(2). DOI: 10.3760/cma.j.issn.1000-6699.2010.02.016 |
| 作者姓名： | 谢云 沙伟伟 周雪 王绍欣 汪玮琳 韩丽萍 王家驰 于德民 |
| 作者单位： | 1. 卫生部激素与发育重点实验室,天津医科大学代谢病医院糖尿病神经内科,300070 2. 北京市昌平区医院 3. 保定市中国人民解放军第252医院 |
| 摘 要： | 观察胰升糖素样肽1(glucagon-like peptide-1,GLP-1)对原代培养的心肌细胞在缺氧/复氧条件下损伤的影响及机制.结果显示,缺氧/复氧使乳酸脱氢酶活性[(210.0±11.5)对(101.4±6.5)U/L]、细胞凋亡率[(8.138±1.512)对(0.575±0.168)%]和半胱氨酸天冬氨酸蛋白酶3活性[(44.52±5.69)对(19.98±1.97),均P<0.01]均升高,GLP-1能直接作用心肌细胞,并对其在缺氧/复氧的损伤具有抑制作用[乳酸脱氢酶活性(190.2±9.0)U/L,细胞凋亡率(2.688±0.580)%,半胱氨酸天冬氨酸蛋白酶3活性30.34±4.18],该作用可能与磷脂酰肌醇3激酶(PBK)/Akt介导的抗细胞凋亡作用有关. |
| 关 键 词： | 胰升糖素样肽1 缺氧复氧 细胞凋亡 半胱氨酸天冬氨酸蛋白酶3 |
The mechanism of the effect of glucagon-like peptide-1 on injury to neonatal rat cardiomyocytes induced by hypoxia-reoxygenation |
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| XIE Yun,SHA Wei-wei,ZHOU Xue,WANG Shao-xin,WANG Wei-lin,HAN Li-ping,WANG Jia-chi,YU De-min. The mechanism of the effect of glucagon-like peptide-1 on injury to neonatal rat cardiomyocytes induced by hypoxia-reoxygenation[J]. Chinese Journal of Endocrinology and Metabolism, 2010, 26(2). DOI: 10.3760/cma.j.issn.1000-6699.2010.02.016 | |
| Authors: | XIE Yun SHA Wei-wei ZHOU Xue WANG Shao-xin WANG Wei-lin HAN Li-ping WANG Jia-chi YU De-min |
| Abstract: | To study the possible mechanism of the effect of glucagon-like peptide-1 (GLP-1) on injury to neonatal rat cardiomyocytes induced by hypoxia-reoxygenation. Lactate dehydrogenase activity [(210.0±11.5) vs (101.4±6.5) U/L] ,apoptosis rate [(8. 138±1. 512) vs(0. 575±0. 168)%] ,and caspase-3 activity [(44.52± 5.69)vs(19.98±1.97) ,all P<0.01] were all increased after hypoxia-reoxygenation. GLP-1 appears to directly act on cardiomyocytes and to protect them from hypoxia-reoxygenation injury [lactate dehydrogenase (190.2±9.0) U/ L, apoptosis rate (2.688±0.580) %, caspase-3 activity 30.34±4.18] mainly by inhibiting the apoptosis probably via the PBK/Akt signaling pathways. |
| Keywords: | Glucagon-like poptide-1 Hypoxia-reoxygenation Apoptosis Caspase-3 |
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