siRNA干扰ABCG2影响肝癌细胞的耐药及侵袭能力

目的探讨ABCG2在肝癌细胞的耐药及转移侵袭过程中的作用。方法 MTT方法检测siRNA干扰前后三种肝癌细胞MHCC97-L、MHCC97-H、SMMC-7721对多柔比星的药物敏感性;划痕与Transwell实验证实这几种肝癌细胞在siRNA干扰前后的转移、侵袭能力;western-blot、PCR实验证实siRNA转染效率。结果 SMMC-7721、MHCC97-L、MHCC97-H细胞内ABCG2水平呈逐渐上升趋势,对多柔比星的耐受程度也同样逐步上升,同时侵袭能力逐渐增强;siRNA干扰后,MHCC97-L、MHCC97-H两株细胞中ABCG2水平明显降低,对多柔比星敏感性明显增强,侵袭能力明显下降。结论 ABCG2不仅在肝癌细胞的耐药过程中起到重要作用,同时还影响肝癌细胞的转移侵袭能力,靶向干扰ABCG2水平增加了化疗药物敏感性并降低肝癌细胞的转移侵袭能力。

ABCG2 affects the chemoresistance and invasion ability in HCC after interfered by siRNA

Objective To determine the relationship between the potential as determined by MTlr assay while the migration and invasion ability was measured by wound-healing assay and transwell assay, respectively. Results Of all the three HCC cell lines, SMMC-7721, MHCC97-L and MHCC97-H showed ascending ABCG2 expression, at the same time, their resistance to doxorubicin and invasion ability increased. After we interfered the ABCG2 level, the ABCG2 overexpression cell lines MHCC97-L and MHCC97-H be- came more sensitive to doxorubicin and their invasion ability weakened. Conclusions ABCG2 not only gives rise to the ehemoresis- tance in HCC but also affects its invasion ability. ABCG2 targeted therapy might increase the sensitivity of HCC chemotherapeutic drugs and reduce the migration and invasion ability.