全氟异丁烯急性肺水肿小鼠模型的建立

目的明确全氟异丁烯急性中毒的剂量-反应关系与其半数致死浓度,建立全氟异丁烯中毒肺水肿动物模型,为进一步研究全氟异丁烯致肺损伤机制及其防治手段奠定基础。方法采用小鼠全身暴露静态吸入全氟异丁烯方式染毒,剂量分别为106、211、316、421、526 mg/m3,染毒5 min,随即观察动物进食、活动及死亡情况。应用加权回归法计算小鼠全氟异丁烯染毒的半数致死浓度。另外,染毒剂量为263 mg/m3,染毒时间为5 min时,测定小鼠染毒前及染毒后2、4、8、12、16、24 h各实验点的肺湿干比变化及支气管肺泡灌洗液中总蛋白含量的变化。结果小鼠全氟异丁烯染毒5 min的半数致死浓度为235 mg/m3,95%的可信限为(136～408)mg/m3。小鼠染毒后8、12、16 h的肺湿干比与对照组相比有显著性差异。染毒后8和12 h的支气管肺泡灌洗液中总蛋白含量与其他各组均有显著性差异。结论本实验确定了小鼠全氟异丁烯染毒5 min的半数致死浓度,并依此成功建立了全氟异丁烯中毒的肺水肿模型。

The Dose-response Relationship of Acute Perfluoroisobutylene Poisoning and Mice Model Establishment of Pulmonary Edema

Objective To determine the dose-response relationship of perfluoroisobutylene acute poisoning and 50% lethal concentration,and to establish a model of pulmonary edema rats,so as to find out more lung injury and treatment of perfluoroisobutylene.Methods Mice were exposed to gaseous perfluoroisobutylene for 5 minutes in a static whole-body exposure inhalation system.The dose was 106,211,316,421,526 mg/m3.The weighted regression method was used to calculate the 50% lethal concentration.The total protein concen...