| 2′,5,6′,7-四羟基二氢黄酮醇对内毒素诱导巨噬细胞活化的影响 |
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| 引用本文： | 葛迪,史清海,伏建峰,刘正祥,郑江.2′,5,6′,7-四羟基二氢黄酮醇对内毒素诱导巨噬细胞活化的影响[J].解放军药学学报,2011(5):427-430. |
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| 作者姓名： | 葛迪 史清海 伏建峰 刘正祥 郑江 |
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| 作者单位： | 兰州军区乌鲁木齐总医院全军临床检验诊断中心;第三军医大学西南医院综合实验研究中心; |
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| 基金项目： | 新疆军区医院人才基金(联卫〔2009〕62号)资助 |
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| 摘 要： | 目的 检测2′,5,6′,7-四羟基二氢黄酮醇对细菌内毒素诱导的巨噬细胞RAW264.7释放TNF-α、IL-6及′TNF-α mRNA表达的影响.方法 RAW264.7细胞经不同浓度(0、10、20、40、80、160 mg/L)2′,5,6 ′,7-四羟基二氢黄酮醇预处理后,ELISA法分别检测LPS( 100 ...
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| 关 键 词： | 2′ 5 6′ 7-四羟基二氢黄酮醇 内毒素 巨噬细胞 TNF-α IL-6 |
The Effect of 2',5,6',7-tetrahydroxyflavanonol Pretreatment on the Activation of LPS-induced Macrophages |
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| GE Di①,SHI Qing-hai①,FU Jian-feng①,LIU Zheng-xiang①,ZHENG Jiang.The Effect of 2',5,6',7-tetrahydroxyflavanonol Pretreatment on the Activation of LPS-induced Macrophages[J].Pharmaceutical Journal of Chinese People's Liberation Army,2011(5):427-430. |
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| Authors: | GE Di① SHI Qing-hai① FU Jian-feng① LIU Zheng-xiang① ZHENG Jiang |
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| Institution: | ② ①Clinical Laboratory Diagnostic Center,Urumqi General Hospital,Lanzhou Military Region,Urumqi 830000,China; ②Medical Research Center,Southwest Hospital,Third Military Medical University,Chongqing 400038,China |
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| Abstract: | Objective To determine the effect of 2′,5,6′,7-tetrahydroxyflavanonol pretreatment on the release of cytokine（TNF-α and IL-6） and the expression of TNF-α mRNA in RAW264.7 cells after exposure to LPS.Methods RAW264.7 cells were pretreated with THF at the indicated dose（0,10,20,40,80,160 mg/L） and stimulated with LPS（100 μg/L）.Meanwhile,ELISA and Real-Time PCR were used respectively to measure the release of cytokine（TNF-α and IL-6） and detect the expression of TNF-α mRNA in RAW264.7 cells.The cytotoxicity of THF on RAW264.7 was established using the MTT assay in order to remove the effect of THF pretreatment on cell viability.Results THF pretreatment at different concentrations significantly decreased the release of TNF-α and IL-6 in LPS-induced RAW264.7 cells in a dose-dependent manner.Furthermore,THF reduced the expression of TNF-α mRNA in LPS-induced RAW264.7 cells in a dose-dependent manner.MTT results showed that THF treatment below 320 mg/L concentration did not affect RAW264.7 cell viability.Conclusion THF can antagonize LPS stimulation on the effects of inflammation cells and is expected to be a candidate prodrug for anti-sepsis. |
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| Keywords: | 2′ 5 6′ 7-tetrahydroxy-flavanonol lipopolysaccharide macrophage TNF-α IL-6 |
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