| 含中药血清对人激素非依赖性前列腺癌细胞增殖的影响 |
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| 引用本文： | 陈晓峰,周芳坚,刘卓炜,韩辉,秦自科,余绍龙,李永红,侯国良. 含中药血清对人激素非依赖性前列腺癌细胞增殖的影响[J]. 临床泌尿外科杂志, 2008, 23(7): 540-543 |
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| 作者姓名： | 陈晓峰 周芳坚 刘卓炜 韩辉 秦自科 余绍龙 李永红 侯国良 |
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| 作者单位： | 1. 湖南省郴州市第一人民医院泌尿外科
2. 华南肿瘤学国家重点实验室,中山大学肿瘤防治中心泌尿外科,广州,510060 |
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| 摘 要： | 目的:探讨不同中药复方水煎剂的大鼠含药血清对人激素非依赖性前列腺癌细胞系PC-3增殖的体外影响.方法:以高剂量(10倍于大鼠常规剂量)的中药复方S、T、F水煎剂对成年雄性去势SD大鼠灌胃(中药组,即S组、T组、F组),每天2次,共3天.另以与中药水煎剂等量的生理盐水灌胃(对照组)进行对照.末次灌胃后于60～90 min时间段内取下腔静脉血制成血清,用以体外培养PC-3,用MTT法测定培养4、24、48、72 h的细胞存活率,比较细胞存活率的差异.结果:中药组和对照组细胞的光密度随培养时间延长均呈增加趋势.培养4 h,各中药组细胞存活率均升高,相互间比较差异无统计学意义.培养24、48 h以后,F组、T组细胞存活率分别开始出现下降.培养4、24、48、72 h,S组细胞存活率均升高,各时间段间差异无统计学意义.结论:中药复方F含药血清抑制PC-3细胞增殖,可用于动物实验进一步明确体内有无抗肿瘤作用;中药复方S促进细胞增殖.
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| 关 键 词： | 前列腺癌 血清药理学 中药复方 含药血清 细胞增殖 |
The effect of serum containing compound recipe on proliferation of human hormone-independent prostate cancer cell |
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| CHEN Xiaofeng,ZHOU FangJian,LIU Zhuowei,HAN Hui,QING Zike,YU Shaolong,LI Yonghong,HOU Guoliang. The effect of serum containing compound recipe on proliferation of human hormone-independent prostate cancer cell[J]. Journal of Clinical Urology, 2008, 23(7): 540-543 |
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| Authors: | CHEN Xiaofeng ZHOU FangJian LIU Zhuowei HAN Hui QING Zike YU Shaolong LI Yonghong HOU Guoliang |
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| Affiliation: | 1Department of Urology;Cancer Center;Sun Yat-sen University;Guangzhou;510060;China;Now Working at Chenzhou No 1 People's Hospital;Hunan |
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| Abstract: | Objective:The present analysis was designed to assess the effect of serum containing compound recipe(CR) on proliferation of hormone-independent prostate cancer(HIPC) cell line PC-3 in Vitro.Methods:The adult male castrated SD rats in CR S,T,and F group were gavaged with decoction of CR S,CR T,and CR F twice a day for 3 days,respectively.The dosage was 10 times of the equivalent dosage calculated according to that of human being.The animals in control group were gavaged with normal saline in the same volume... |
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| Keywords: | PC-3 |
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