The susceptibility of cell lines ofAedes aegypti (Linn.),Aedes albopictus (Skuse) andAedes pseudoscutellaris (Theobald) to infection with Bluetongue virus

Summary Bluetongue virus multiplied in cell lines derived fromAedes albopictus andAedes pseudoscutellaris cells. Virus reached a maximum titre in theAe. pseudoscutellaris cells three days post inoculation, and inAe. albopictus cells six days p. i. Virus growth was demonstrated in both cell lines at 27° C and 37° C. Significant titres of virus were still present in theAe. albopictus cells after five subcultures at 27° C over a period of six weeks. No cytopathic effect was observed in either cell line.A third cell line derived from the mosquitoAe. aegypti did not support the growth of Bluetongue virus.With 1 Figure