甘氨酸延伸型胃泌素对人胃癌细胞株SGC-7901的体外作用

目的体外研究甘氨酸延伸型胃泌素(Glycin-extended gastrin, G-Gly)对人胃癌细胞株SGC-7901增殖以及对丝裂霉素（MMC）促凋亡作用的影响，探讨G-Gly在胃癌发生发展中的作用。方法应用MTT法检测不同浓度G-Gly对SGC-7901增殖的影响，应用流式细胞术检测细胞的凋亡率。结果G-Gly能促进SGC-7901的增殖，其增殖率在一定范围内与剂量有关，在浓度0.1 nmol/L时具有最大增殖效果。其效应不能被缩胆囊肽B受体（Cholecystokinin B receptor，CCKBR）的抗体所抑制。G-Gly能抑制MMC诱导的凋亡（P<0.01）。结论G-Gly能促进SGC-7901的增殖和抑制MMC诱导的凋亡，提示G-Gly能促进胃癌肿瘤的生长。

Effect of Glycin-extended Gastrin on Human Gastric Cancer Cell Line SGC-7901 in Vitro

Objective To explore the effect of Glycin-extended gastrin on proliferation and anti- apoptotic effect of gastric cancer cell line SGC-7901,as well as the role of G-Gly in the development of gastric cancer． Methods Cell proliferation was detected by MTT colorimetric assay at various concentrations of G-Gly，and cell apoptotic rate was examined with flow cyometry analysis． Results G-Gly can promote cell proliferation of SGC-7901 in a dose-dependant manner within a certain extent. The concentration of maximum effect is 0.1 nmol/L.Its effects can not be inhibited by antibody of CCKBR. MMC can promote SGC-7901 cell line apoptosis significantly,but G-Gly could inhibit its apoptosis induced by MMC. Conclusion G-Gly promoted cell proliferation and inhibited cell apoptosis induced by MMC． These results suggest that G-Gly may promoted the development of gastric cancer．