The effect of colchicine and low-dose methotrexate on intestinal ischemia/reperfusion injury in an experimental model |
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Authors: | Ö zlem Boybeyi,Yasemin Dere Gunal,Pinar Atasoy,Ucler Kısa,Mustafa Kemal Aslan |
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Affiliation: | 1. K?r?kkale University, Medical Faculty, Department of Pediatric Surgery, K?r?kkale, Turkey;2. K?r?kkale University, Medical Faculty, Department of Pathology K?r?kkale, Turkey;3. K?r?kkale University, Medical Faculty, Department of Biochemistry, K?r?kkale, Turkey |
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Abstract: | AimIntestinal ischemia/reperfusion (I/R) injury is a serious clinical condition. Colchicine and low-dose methotrexate have anti-inflammatory features. An experimental model was conducted to investigate the effect of colchicine and methotrexate on intestinal I/R injury.MethodsTwenty-four rats were included. Only laparotomy was done in control group (CG, n = 6). In experimental groups, superior mesenteric artery was occluded. After 1 h ischemia, reperfusion (1 h) was started by de-occlusion. 30 min before reperfusion, saline in sham group (SG, n:6), colchicine (1 mg/kg) in colchicine group (CNG, n:6), and methotrexate (0.1 mg/kg) in methotrexate group (MTXG, n:6) were infused intraperitoneally. Small intestines were harvested for evaluation of intestinal mucosal injury (Chiu score) and oxidative stress markers (nitric oxide: NO, malondialdehyde: MDA, superoxide dismutase: SOD).ResultsBiochemically, MDA levels were significantly low in CG compared to SG, CNG, and MTXG (p < 0.05). NO levels were significantly low and SOD levels were significantly high in CG compared to MTXG (p < 0.05). Histopathologically, Chiu score was significantly low in CG compared to SG, CNG, and MTXG (p < 0.05), and significantly high in MTXG compared to SG and CNG (p < 0.05).ConclusionThe present experimental model caused I/R injury in rat intestines. Contrary to literature, it was found that methotrexate worsens and colchicine does not attenuate intestinal I/R injury. |
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Keywords: | Intestinal ischemia Ischemia/reperfusion injury Colchicine Methotrexate Experimental |
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