| VD_3结合蛋白的分离纯化 |
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| 引用本文： | 单友梅,吴国球,余建华,高茂馗.VD_3结合蛋白的分离纯化[J].东南大学学报(医学版),2001,20(1). |
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| 作者姓名： | 单友梅 吴国球 余建华 高茂馗 |
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| 作者单位： | 东南大学附属中大医院检验科, |
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| 摘 要： | 目的 :纯化VD3 结合蛋白 (DBP)。方法 :利用DBP的相对分子质量及电荷性质 ,分别用阳离子交换剂、阴离子交换剂和凝胶过滤等分离目的蛋白 ,SDS PAGE鉴定纯度 ,并进行活性分析。结果 :分离到相对分子质量为 5 80 0 0的蛋白质 ,SDS PAGE呈单一区带。结论 :成功分离纯化出DBP。
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| 关 键 词： | 结合蛋白 分离 纯化 |
Separation and Purification of VD_3 Binding Protein |
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| SHAN You-mei,WU Guo-qiu,YU Jian-hua,GAO Mao-kui.Separation and Purification of VD_3 Binding Protein[J].Journal of Southeast Univ: Medical Sci Ed,2001,20(1). |
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| Authors: | SHAN You-mei WU Guo-qiu YU Jian-hua GAO Mao-kui |
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| Abstract: | Objective To separate and purify VD 3 binding protein(DBP).Methods The protein was purified by chromatographers of SP Sephadex,QAE cellulose,Sepharose 4B,Sephadex G200 according to its characteristics of molecular weight and electric charge.Purity,activity and the concentration of SOP were investigated by SDS PAGE.Result The protein of 58?000 was obtained and was indicated single band by SDS PAGE.Conclusion VD 3 binding protein was separated and purified successfully. |
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| Keywords: | VD 3 binding protein separation purification |
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