12-脂氧合酶在胰腺癌中的表达及其临床意义 |
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引用本文: | 龚镭,胥明,彭晓斌,唐学军,朱颖伟,陆健.12-脂氧合酶在胰腺癌中的表达及其临床意义[J].中华胰腺病杂志,2011,11(6). |
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作者姓名: | 龚镭 胥明 彭晓斌 唐学军 朱颖伟 陆健 |
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作者单位: | 南京医科大学附属无锡市第二人民医院消化内科,无锡,214002 |
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摘 要: | 目的 检测胰腺癌组织和胰腺癌细胞株SW1990和PANC1的12-脂氧合酶(12-lipooxygenase,12-LOX)表达,探讨其与胰腺癌临床病理参数的关系.方法 分别应用免疫组化染色、RT-PCR和蛋白质印迹法检测胰腺癌组织、癌旁正常胰腺组织及胰腺癌细胞株SW1990和PANC1中12-LOX mRNA和蛋白的表达.分析胰腺癌组织12-LOX表达与临床病理参数的相关性.结果 30例胰腺癌组织12-LOX表达阴性8例,弱阳性7例,强阳性15例,总阳性率为73.3%.SW1990和PANC1细胞均表达12-LOX.阳性表达的胰腺癌组织及两株胰腺癌细胞株均表达12-LOX mRNA,而癌旁正常胰腺组织不表达12-LOX mRNA及蛋白.胰腺癌组织12-LOX强阳性表达与肿瘤TNM分期、肿瘤病理分级和淋巴结转移相关(P<0.05),而与患者年龄、性别无关.结论 12-LOX在胰腺癌中表达上调,与胰腺癌的恶性生物学行为有关.
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关 键 词: | 胰腺肿瘤 12-脂氧合酶 病理学 免疫组织化学 逆转录聚合酶链反应 |
Expression and clinical significance of lipoxygenase-12 in human pancreatic adenocarcinoma |
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Authors: | GONG Lei X Ming PENG Xiao-bing TANG Xue-jun ZHU Ying-wei LU Jian |
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Institution: | GONG Lei,X(U) Ming,PENG Xiao-bing,TANG Xue-jun,ZHU Ying-wei,LU Jian |
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Abstract: | Objectives To study the expression of lipoxygenase-12 (12-LOX) in human pancreatic adenocarcinoma and its relationship with the clinicopathological parameters.Methods Expression of 12-LOX in pancreatic carcinoma tissue,pancreatic cancer cell lines SW1990 and PANC1,and adjacent normal pancreatic tissue was detected by using immunohistochemistry,RT-PCR,and Western blot,respectively.The relationship between 12-LOX expression and clinicopathological parameters was analyzed.Results Expression of 12-LOX was negative in 8 cases,weak positive in 7 cases and strong positive in 15 cases of pancreatic carcinoma.The overall positive rate in pancreatic carcinoma was 73.3%.The expressions of 12-LOX mRNA and protein were positive in pancreatic cancer cell lines SW1990 and PANC1,and negative in adjacent normal pancreatic tissue.The strong positive expression of 12-LOX in pancreatic cancer was associated with TNM stage,pathological grade,lymph node metastasis (P < 0.05 ).Conclusions The expression of 12-LOX was up-regulated in pancreatic cancer and is correlated well to malignant behaviors of tumor. |
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Keywords: | Pancreatic neoplasms 12-Lipoxygenase Pathology Immunohistochemistry RT-PCR |
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