乳腺上皮增生性病变与乳腺癌的免疫组化研究

对24例乳腺良性增生性病变与11例乳腺导管癌标本、采用细胞角蛋白、肌动素及 S-100蛋白免疫组化染色进行鉴别。结果示细胞角蛋白标记良性上皮细胞及癌细胞均阳性,而肌上皮细胞阴性;肌动素标记上皮和癌细胞均阴性,良性肌上皮细胞阳性,而癌灶肌上皮细胞阴性;S-100蛋白与肌动素结果大致相同。故认为鉴别乳腺良、恶性病变以采用高分子量细胞角蛋白和肌动素抗体为好。

Studies on epithelial proliferative lesions and carcinoma of the breast by immunohistochemistry

Epitheliosis of the breast should be distinguished from ductal carcinoma of the breast.In present study,a series of 24 benign breast epithelial proliferative lesions and 11 ductal carcinomas were examined using immunohistochemical method with cytokeratin(CK),actin and S-100 protein. The results showed positive immunostaining in benign epithelial proliferating cells and carcinoma cells while negative in myoepithelial cells for CK.Muscle specific actin was demonstrable in myoepithelial cells of benign groups,and negative in benign epithelial cells and carcinoma cells.A similar but weakly immunoreaetivity was showed for S-100 protein compared with actin.The former was not as sensitive and evident as the latter.It was suggested that high molecular weight cytokeratin and actin may serve as a nseful marker in differentiating benign proliferative lesions and ductal carcinoma of the breast.