胃癌组织中SEPT9_i1和PLZF的表达及其临床意义

目的 探讨SEPT9亚型1蛋白（SEPT9_i1）和早幼粒细胞白血病锌指蛋白（PLZF）在胃癌组织中的表达水平，分析两者与胃癌临床病理特征及预后的关系。方法 采用免疫组化EnVision二步法检测75例胃癌组织和62例癌旁组织石蜡切片中SEPT9_i1和PLZF表达情况，以阳性细胞百分比与着色强度乘积值分为低表达（＜5分）和高表达（≥5分），分析两者表达与胃癌临床病理参数（性别、年龄、临床分期、肿瘤部位、Lauren分型、肿瘤大小、分化程度、浸润深度和淋巴结转移）及预后的关系；同时采用Spearman相关分析胃癌组织中SEPT9_i1和PLZF表达的相关性。结果 胃癌组织SEPT9_i1高表达率高于癌旁组织（65.33％ vs. 37.10％， P＜0.05），而PLZF高表达率低于癌旁组织（41.33％ vs. 85.48％， P＜0.05）；胃癌组织中SEPT9_i1与PLZF的表达呈负相关（r=-0.299， P=0.009），且两者表达与临床分期及淋巴结转移均有关，此外SEPT9_i1与浸润深度有关，而PLZF与分化程度有关（P＜0.05）。SEPT9_i1高表达者的中位总生存期（OS）为21.5个月，低于低表达者的32.0个月（P＜0.05）；PLZF高表达者的中位OS为26.5个月，与低表达者31.0个月的差异无统计学意义（P＞0.05）。结论 SEPT9_i1在胃癌组织中高表达，PLZF为低表达，两者均与临床分期及淋巴结转移有关，且SEPT9_i1表达与胃癌患者预后有关，提示两者可能在胃癌的发生发展中起重要作用，可用于辅助胃癌的诊断和病情评估。

Expression and clinical significance of PLZF and SEPT9_i1 in gastric cancer

Objective To investigate the expression level of Septin 9 isoform 1（SEPT9_i1）and promyelocytic leukaemia zinc finger（PLZF）protein in gastric cancer tissues， and to analyze the relations between them with the clinical pathological features and prognosis of gastric cancer. Methods The immunohistochemistry EnVision two step method was used to detect the SEPT9_i1 and PLZF expression in paraffin embedded 75 cases of gastric cancer tissues and 62 cases of paracancerous tissue. The staining Results of the tumors were expressed as the product of the staining intensity and the percentage of positive tumor cells. The expression was divided into high expression group（＜5）and low expression group（≥5）. We analyzed the correlation between tumor tissue expression and clinical parameters， such as gender， age， clinical stage， tumor location， Lauren typing， tumor size， differentiation， depth of invasion and lymph node metastasis. Moreover， the correlation between tumor tissue expression and prognosis were also analyzed. The Spearman correlation analysis was used to analyze the expression of PLZF and SEPT9_i1 in gastric cancer tissues. Results The higher expression of SEPT9_i1 in gastric cancer tissues was higher than that in para-carcinoma tissues（65.33％ vs. 37.10％， P＜0.05）， and the PLZF expression was lower than that in para-carcinoma tissues（41.33％ vs. 85.48％， P＜0.05）. There was negative correlation between expression of SEPT9_i1 and PLZF in gastric cancer tissues（P=0.009， r=-0.299）. The expression of both proteins was related to clinical stage and lymph node metastasis. Expression of SEPT9_i1 was related to the depth of invasion， and the expression of PLZF was related to the differentiation degree（P＜0.05）. The median overall survival（OS）was 21.5 months in patients with high expression of SEPT9_i1， lower than 32.0 months of patients with low expression of SEPT9_i1（P＜0.05）. The median OS was 26.5 months in patients with high expression of PLZF， similar to 31.0 months in patients with low expression of PLZF（P＞0.05）. Conclusion SEPT9_i1 was highly expressed in gastric cancer tissues but PLZF was low expression. Both were related to clinical stage and lymph node metastasis, and SEPT9_i1 expression is related to the prognosis of gastric cancer patients，suggesting that they may play an important role in the occurrence and development of gastric cancer，and can be used in the diagnosis and evaluation of gastric cancer.