Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization. |
| |
Authors: | D C Foster C A Sprecher F J Grant J M Kramer J L Kuijper R D Holly T E Whitmore M D Heipel L A Bell A F Ching et al. |
| |
Affiliation: | D C Foster, C A Sprecher, F J Grant, J M Kramer, J L Kuijper, R D Holly, T E Whitmore, M D Heipel, L A Bell, A F Ching, et al. |
| |
Abstract: | Thrombopoietin (TPO), a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Recently we have isolated a cDNA encoding a ligand for the murine c-mpl protooncogene and shown it to be TPO. By employing a murine cDNA probe, we have isolated a gene encoding human TPO from a human genomic library. The TPO locus spans over 6 kb and has a structure similar to that of the erythropoietin gene (EPO). Southern blot analysis of human genomic DNA reveals a hybridization pattern consistent with a single gene locus. The locus was mapped by in situ hybridization of metaphase chromosome preparations to chromosome 3q26-27, a site where a number of chromosomal abnormalities associated with thrombocythemia in cases of acute myeloid leukemia have been mapped. A human TPO cDNA was isolated by PCR from kidney mRNA. The cDNA encodes a protein with 80% identity to previously described murine TPO and is capable of initiating a proliferative signal to murine interleukin 3-dependent BaF3 cells expressing the murine or human TPO receptor. |
| |
Keywords: | |
|
|