补肾益精方对去势大鼠椎体结构、成分与生物力学性能的影响

目的:评价补肾益精方提高去势大鼠椎体骨质量的有效性,探讨本方改善模型松质骨生物力学性能的作用机制.方法:40只12月龄Wistar雌性大鼠随机分为正常对照组、模型对照组、补肾益精方组与倍美力组,每组10只.给药组的给药时点是双侧卵巢切除术后90 d,给药组与非给药组的处死时点是卵巢切除术或假切除术后180 d.处死以后,分别测定各组大鼠第二腰椎宏观几何结构参数、生物化学成分参数、生物力学性能参数.结果:模型对照组的腰椎宏观几何结构参数与正常对照组比较,未见显著性差异(P>0.05);补肾益精方组、倍美力组与模型对照组比较,也均未见显著性差异(P>0.05).模型对照组的生物化学成分参数与正常对照组比较,其中骨矿盐密度显著减少(P<0.05),骨胶原密度减少不显著(P>0.05);补肾益精方组、倍美力组与模型对照组比较,补肾益精方组的骨矿盐密度与骨胶原密度分别上升3%(P<0.05)和8%(P>0.05),倍美力组分别上升9%(P<0.05)和12%(P>0.05).模型对照组的结构力学参数以及材料力学参数都显著低于正常对照组(P<0.05);尤其在弹性阶段的结构力学参数与材料力学参数方面,补肾益精方组比模型对照组显著升高(P<0.05),但是倍美力组升高不显著(P>0.05);在塑性阶段的结构力学参数与材料力学参数方面,补肾益精方组、倍美力组与模型对照组比较均明显升高(P<0.05),两者升高的幅度分别是30.3%与25.8%.结论:补肾益精方不仅提高去势大鼠椎体的骨质量,改善模型椎体弹性阶段的力学性能的作用也相对优于倍美力,具体机制有待进一步研究.

Effect of Bushen Yijing Recipe on Structure,Components and Biomechanical Properties of Cancellous Bone of Vertebrate in Osteoporotic Rat Model Induced by Ovariectomy

Objective: To evaluate the efficacy of Bushen Yijing Recipe (BSYJR) in improving the quality of cancellous bone of vertebrate in osteoporotic rat model induced by ovariectomy, and inquiry into the mechanism in improving biomechanical properties of cancellous bone.Methods: Forty 12 month old female Wistar rats were divided randomly into Sham control group, Ovariectomy (Ovx) control group, BSYJR group and Premarin group.Ten rats were in each group. The time point of operation was at 12 month age.The time piont of administering rats drug was at 15 month old.The time point of stopping giving drug and sacrificing the rats in the four groups was at 18 month old.After sacrifice, macroscopic geometry structure (height and volume), chemical components (bone mineral density (BMD) and bone collagen density (BCD)) and biomechanical properties of compression (structural mechanical property and material mechanical property) of No. 2 lumbar vertebrate were determined. Results: The macroscopic geometry structure in Ovx control group was not altered significantly (P>0.05), respectively vs sham control group; in BSYJR group and Premarin group, the macroscopic geometry structure was not altered significantly (P>0.05), respectively vs Sham control group. In chemical components, the BMD and the BCD in Ovx control group were reduced by 11% (P<0.05) and 6% (P>0.05), respectively vs Sham control group. In comparison with those of Ovx control group, the BMD and the BCD in BSYJR group were increased by 3% (P<0.05) and 8% (P>0.05), and the BMD and the BCD in Premarin group were increased by 9% (P<0.05) and 12% (P>0.05). The parameters of structural mechanical property (maximum load, elastic load and elastic deformation of collagen) and the parameters of material mechanical property (maximum bone strength, elastic bone strength and elastic deformation strength of collagen) were all decreased significantly (P<0.05), respectively vs Sham control group. Especially, in elastic phase, the parameters of structural mechanical property (elastic load and elastic deformation of collagen) and the parameters of material mechanical property (elastic bone strength and elastic deformation strength of collagen) in BSYJR group were increased significantly (P<0.05),vs Ovx control group; but those in Premarin group were not increased significantly (P>0.05), vs Ovx control group. In plastic phase, the parameters of structural mechanical property (maximum load) and the parameters of material mechanical property (maximum bone strength) were increased by 30.3% (P<0.05) and 48.2% (P<0.05), respectively vs Ovx control group. Conclusion: BSYJR can not only improve the quality of cancellous bone of vertebrate in the rat model induced by ovariectomy, but also have superiority to Premarin in improving the cancellous bone mechanical property in elastic phase.