| 藤梨根提取物对人胃癌SGC-7901细胞凋亡及Bcl-2的影响 |
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| 引用本文： | 白吉庆,王小平,叶峥嵘.藤梨根提取物对人胃癌SGC-7901细胞凋亡及Bcl-2的影响[J].陕西中医学院学报,2012,35(1):59-61. |
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| 作者姓名： | 白吉庆 王小平 叶峥嵘 |
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| 作者单位： | 陕西中医学院,陕西咸阳,712046 |
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| 基金项目： | 陕西省教育厅资助项目(08JK266);陕西省科技厅中药现代化资助项目[2009K19-03(2);2010K17-02] |
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| 摘 要： | 目的研究藤梨根提取物对人胃癌SGC-7901细胞凋亡的影响及其部分作用机理。方法采用不同浓度的藤梨根提取物作用于人胃癌SGC-7901细胞,流式细胞仪检测细胞凋亡,免疫组织化学方法检测Bcl-2基因表达。结果藤梨根作用于SGC-7901,在(8～32)mg/mL时促进该细胞呈不同程度的凋亡;藤梨根提取物大、中、小剂量组对人胃癌SGC-7901细胞Bcl-2蛋白表达阳性灰度值分别为103.41±1.01,104.05±1.239,0.52±1.09。结论藤梨根提取物对SGC-7901有明显促凋亡作用;藤梨根提取物抑制SGC-7901 Bcl-2蛋白水平的表达有可能是促进SGC-7901凋亡的作用机制。
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| 关 键 词： | 藤梨根 提取物 胃癌 Bcl-2 |
Influence of Tengligen(Actinidiae) Extracts on the Apoptosis of SGC-7901 and Bcl-2 |
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| Bai Jiqing
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Wang Xiaoping
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Ye Zhengrong.Influence of Tengligen(Actinidiae) Extracts on the Apoptosis of SGC-7901 and Bcl-2[J].Journal of Shaanxi College of Traditional Chinese Medicine,2012,35(1):59-61. |
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| Authors: | Bai Jiqing Wang Xiaoping Ye Zhengrong |
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| Institution: | Bai Jiqing Wang Xiaoping Ye Zhengrong (Shaanxi University of Chinese Medicine,Xianyang,Shaanxi,712046) |
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| Abstract: | Objective To study the influence of Tengligen(Actinidiae) extracts on the apoptosis of SGC-7901 and part of its mechanism.MethodTengligen(Actinidiae) extracts of different concentrations were applied to SGC-7901.Flow cytometry(FCM) was employed to test the apoptosis and immunohistochemistry(IHC) method was adopted to test the gene expression of Bcl-2.ResultsTengligen(Actinidae) acts on SGC-7901 and to some extent promotes the apoptosis with the concentration of 8～32 mg· mL-1.The positive expression gray value of Tengligen(Actinidae) extracts of large,middle and small dosage groups on Bcl-2 protein of human cancer cells SGC-7901 are respectively 103.41±1.01,104.05±1.23 and 90.52±1.09.ConclusionTengligen(Actinidae) extracts has significant apoptosis-stimulation function on SGC-7901;The inhibition function of Tengligen(Actinidae) extracts on the expression of SGC-7901 Bcl-2 might be the mechanism of promoting apoptosis of SGC-7901. |
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| Keywords: | Tengligen(Actinidae) extracts gastric cancer Bcl-2 |
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