非小细胞肺癌中Per2的表达及其与预后的关系

目的：研究生物钟基因Per2在非小细胞肺癌（NSCLC）中的表达及其与预后的关系。方法：采用免疫组织化学染色法检测60例NSCLC组织和20例正常肺组织中Per2的表达,结合临床病理和随访资料进行分析。结果：在NSCLC组织和正常肺组织中Per2的阳性表达率分别为71.7%（43/60）和95.0%（19/20）,差异有统计学意义（P〈0.05）。NSCLC中Per2的表达缺失与分化程度及TMN分期相关（P〈0.05）,单因素分析结果表明Per2的表达状态与NSCLC的预后相关（P〈0.05）,多因素分析结果显示分化程度、TNM分期、淋巴结转移情况及Per2的表达状态均非影响NSCLC预后的独立危险因素（P〉0.05）。结论：Per2在NSCLC中表达降低,其阴性表达在NSCLC的发生和发展中起重要作用,影响NSCLC的预后。

Expression of circadian gene Per2 in NSCLC and its relation to the prognosis

Objective： To study the expression of circadian gene Per2 in NSCLC and its relation to prognosis. Methods： The expression of Per2 was measured in 60 NSCLC and 20 normal lung tissue by immunohistochem- istry assay, then we analyze the existence and prognosis. Results： The positive expression rates of Per2 in NSCLC and in normal lung tissue were 71.7% and 95.0%（P0.05）. The negative expression of Per2 in NSCLC was correlated with pathological differentiation or TNM stage（P0.05）. Kaplan-Meier analysis showed that the survival rate of 60 NSCLC was significantly affected by pathological differentiation, TNM stage, lymphatic that none of them was independent prognostic factor of NSCLC（P0.05）. Conclusion： The expression of Per2 in NSCLC decreases. The negative expression of Per2 may contribute to the development and invasion in NSCLC, while Per2 may be a beneficial prognosis factor in patients with NSCLC.