消痰散结方药物血清对人胃癌MKN-45细胞增殖和凋亡的影响

目的：观察消痰散结方药物血清对人胃癌MKN-45细胞生长和凋亡的影响。方法：采用低、中、高浓度消痰散结方药物血清处理MKN-45细胞后，倒置相差显微镜下观察MKN-45细胞的形态学变化，血球计数盘计数细胞，细胞活性计数试剂盒检测消痰散结方药物血清对MKN-45细胞增殖的影响；Annexin V-异硫氰酸荧光素（{luorescein isothiocyanate，FITC）／碘化丙啶（propidiumiodide，PI）双标记法流式细胞术检测消痰散结方药物血清诱导MKN-45细胞后细胞凋亡的情况。结果：低、中、高浓度消痰散结方药物血清均能不同程度地抑制人胃癌MKN-45细胞的增殖，细胞发生了脱落、变圆、折光率下降等形态学变化；Annexin V-FITC／PI双标记法检测显示消痰散结方药物血清可诱导细胞发生凋亡，其中中、高剂量的效果有高于低剂量的趋势。结论：消痰散结方药物血清可抑制人胃癌MKN-45细胞的增殖，促进其凋亡。

Effects of Xiaotan Sanjie Decoction-containing serum on proliferation and apoptosis of human gastric cancer cells MKN-45

Objective： To observe the effects of serum containing Xiaotan Sanjie Decoction, a compound traditional Chinese herbal medicine, on proliferation and apoptosis of human gastric cancer ceil line MKN-45. Methods. After coculturing MKN-45 cells with low-, medium- and high-dose Xiaotan Sanjie Decoction-containing serum, inverted microscope was utilized to observe morphological changes and counting chamber was used to count the MKN-45 cells; the proliferation of MKN-45 cells was determined by cell counting kit 8; Annexin V- fluorescein isothiocyanate/propidium iodide double label method was used to detect apoptosis rate of MKN- 45 cells.Results： Xiaotan Sanjie Decoction-containing serum significantly inhibited the proliferation of MKN-45 cells. The typical morphological changes of apoptotic MKN-45 cells were observed after treating with Xiaotan Sanjie Decoction-containing serum, The cell apoptosis was also observed by flow cytometry and the apoptosis rates of medium- and high-dose Xiaotan Sanjie Decoction-containing serum groups were higher than that of low-dose group. Conclusion： Xiaotan Sanjie Decoction-containing serum can inhibit the MKN-45 cell proliferation and induce cell apoptosis, which provides an experimental evidence for its treatment of human gastric cancer.