救顽汤对慢性湿疹模型大鼠作用研究

目的通过观察救顽汤对慢性湿疹大鼠模型皮损、脾脏指数,初步评价救顽汤治疗慢性湿疹作用。方法随机将60只雄性SD大鼠分为6组,每组10只,分别为空白组、模型组、阳性组、救顽汤低剂量组、中剂量组、高剂量组。除空白组外,各组大鼠运用2,4-二硝基氯苯(DNCB)复制慢性湿疹动物模型。模型建立后连续给药10 d,比较各组间皮损程度、脾指数。结果与空白组相比较,模型组大鼠皮损评分显著升高,脾脏指数降低(P<0.01);与模型组相比较,救顽汤高剂量、中剂量组给药后皮损评分明显降低,脾脏指数升高(P<0.01),低剂量组无差异(P>0.05)。结论救顽汤能有效减轻慢性湿疹大鼠模型的皮损程度,降低皮损评分,具有明显的治疗作用。救顽汤能有效升高湿疹模型降低的脾指数,其治疗慢性湿疹的作用机制可能与提高机体免疫有关。

Research on the Effects of Jiuwan Decoction on Chronic Eczema Model Rats

Objective To observe the effects of Jiuwan decoction on skin lesions, spleen in rats model of chronic eczema, the model was established to initially investigate the immune mechanism of Jiuwan decoction in curing chronic eczema. Methods 60 SD rats were randomly divided into 6 groups including blank group, model group, positive group, low-dose, mid-dose and high-dose groups with 10 rats in each group. The experimental groups were used to replicate the animal model of chronic eczema using 2, 4-dinitrochlorobenzene(DNCB) except for the blank group. These rats were doses for 10 days after successful model establishment. The degree of skin lesions and spleen index were compared among these groups. Results Compared with the blank group, the skin lesion score of the model group increased statistically whereas the spleen index decreased significantly( P<0.01). Compared with the model group, the skin lesion score level in the high-and mid-dose groups of Jiuwan decoction decreased significantly, and the spleen index content increased(P<0.01). There was no difference in the low dose group(P>0.05). Conclusion Jiuwan decoction can effectively reduce the degree of skin lesions in the rat model of chronic eczema, reduce the score of skin lesions, and has an obvious therapeutic effect. Jiuwan decoction can effectively increase the spleen index reduced in the eczema model, and its therapeutic mechanism for chronic eczema may be related to the improvement of body immunity.