| 基于SPA-PLS的秦皮提取液中秦皮甲素的快速测定 |
| |
| 引用本文： | 杨铭,陈佳蕾,余敏英,史秀峰,顾希钧,钮慧珏,徐嘉.基于SPA-PLS的秦皮提取液中秦皮甲素的快速测定[J].中成药,2011,33(5):847-851. |
| |
| 作者姓名： | 杨铭 陈佳蕾 余敏英 史秀峰 顾希钧 钮慧珏 徐嘉 |
| |
| 作者单位： | 上海中医药大学附属龙华医院药剂科;复旦大学附属肿瘤医院药剂科; |
| |
| 基金项目： | 上海中医药大学自然科学基金资助项目(0934); 上海市教委高校选拔培养优秀青年教师科研专项基金(5ZY10100) |
| |
| 摘 要： | 目的探讨连续投影算法(SPA)结合偏最小二乘法(PLS)用于快速测定秦皮提取液中秦皮甲素的可行性。方法以HPLC分析值作为参照,采集不同产地的秦皮提取液的紫外光谱,运用SPA算法筛选样本的紫外特征波长,PLS算法建立快速测定秦皮甲素的数学模型。结果应用SPA算法筛选,只用了全部变量的16.21%(65个变量)建立模型,所建模型的预测集相关系数(RP)及预测均方根(RMSEP)分别为0.969 0、0.067 0。结论 SPA算法可以在不降低模型性能的前提下显著降低模型的复杂度,结合PLS算法建立的模型可以用于快速测定秦皮提取液中秦皮甲素。
|
| 关 键 词： | 连续投影算法 偏最小二乘法 紫外光谱 秦皮甲素 |
Fast determination of aesculin in Fraxini Cortex extract by SPA-PLS |
| |
| YANG Ming,CHEN Jia-lei,YU Min-ying,SHI Xiu-feng,GU Xi-jun,NIU Hui-jue,XU Jia.Fast determination of aesculin in Fraxini Cortex extract by SPA-PLS[J].Chinese Traditional Patent Medicine,2011,33(5):847-851. |
| |
| Authors: | YANG Ming CHEN Jia-lei YU Min-ying SHI Xiu-feng GU Xi-jun NIU Hui-jue XU Jia |
| |
| Institution: | YANG Ming1,CHEN Jia-lei1,YU Min-ying1,SHI Xiu-feng1,GU Xi-jun1,NIU Hui-jue1,XU Jia2(1.Medicament Department Of Longhua Hospital Affiliated to Shanghai Univesity of TCM,Shanghai 200032,China 2.Medicament Department Of Tumor Hospital Affiliated to Fudan Univesity,China) |
| |
| Abstract: | AIM:To use successive projections algorithm(SPA) and partial least squares(PLS) to fast determine aesculin of Fraxini Cortex extract from different batches.METHODS Ultraviolet spectroscopy of Fraxini Cortex extract was selected.The characteristic spectral data was deduced using SPA and subjected to PLS model to fast determine aesculin with the reference of HPLC.RESULTS Sixty-five(16.21%) characteristic wavelengths were identified by SPA as significant wavelengths used for determination of aesculin content.W... |
| |
| Keywords: | successive projections algorithm partial least squares ultraviolet spectra aesculin Fraxini Cortex |
| 本文献已被 CNKI 等数据库收录！ |
|
