| 我国不同地区天门冬核DNAITS序列分析 |
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| 引用本文： | 欧立军,张人文,谈智文,赵婷婷,佘朝文.我国不同地区天门冬核DNAITS序列分析[J].中草药,2011,42(7):1402-1406. |
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| 作者姓名： | 欧立军 张人文 谈智文 赵婷婷 佘朝文 |
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| 作者单位： | 1. 湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化,418008;民族药用植物资源研究与利用湖南省重点实验室,湖南怀化,418008;怀化学院生命科学系,湖南怀化,418008
2. 怀化学院生命科学系,湖南怀化,418008 |
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| 基金项目： | 湖南省高校创新平台开放基金项目(09K106); 湖南省科技计划重点项目(2009FJ2008) |
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| 摘 要： | 目的 研究我国不同地区天门冬核DNA ITS序列差异及其与地理位置的关系,为不同居群天门冬的鉴定和道地产区的确定提供理论依据.方法 运用PCR法对25个地区的75个样本ITS序列扩增后双向测序,用软件DNAMAN和MEGA4分析测序结果.结果 每个地区的3个样本的ITS序列基本相同,不同地区样本间的ITS全序列存在一定差异,ITS2片段变异高于ITS1.贵州产天门冬遗传分化度较大,变异位点和信息位点较多.ITS序列构建的系统树表明,同一个地区的3个样本优先聚类,然后是同省的样本聚类,位于北纬24.6°～36.6°和22.2°～24.4°的样本分别聚为1大支;第1大支中包括青海、湖南和贵州省的样本,第2大支则包括浙江、广东、云南和广西4省的样本.结论 ITS序列可鉴定不同产地的天门冬,贵州省是天门冬药材道地产区之一,不同地区天门冬的亲缘聚类主要与纬度相关,与经度关系不大.
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| 关 键 词： | 天门冬 ITS序列 分子鉴定 道地性 聚类分析 |
| 收稿时间： | 2010/12/6 0:00:00 |
Nuclear DNA ITS sequence analysis of Asparagus cochinchinensis from different geographical regions in China |
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| OU Li-jun,ZHANG Ren-wen,TAN Zhi-wen,ZHAO Ting-ting and SHE Chao-wen.Nuclear DNA ITS sequence analysis of Asparagus cochinchinensis from different geographical regions in China[J].Chinese Traditional and Herbal Drugs,2011,42(7):1402-1406. |
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| Authors: | OU Li-jun ZHANG Ren-wen TAN Zhi-wen ZHAO Ting-ting and SHE Chao-wen |
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| Institution: | OU Li-jun1,2,3,ZHANG Ren-wen3,TAN Zhi-wen3,ZHAO Ting-ting3,SHE Chao-wen1,3 1.Key Laboratory of Hunan Higher Education for Hunan-western Medicinal Plant and Ethnobotany,Huaihua 418008,China 2.Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources,China 3.Department of Life Science,Huaihua University,China |
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| Abstract: | Objective The nuclear DNA ITS sequence variation and its correlation to geographical distribution of Asparagus cochinchinensis from different populations in China were studied,the aims were to reveal the difference among different populations and determine the genuineness of A.cochinchinensis.Methods The ITS fragments of 75 samples from 25 populations were amplified and bidirectional sequenced.And they were analyzed using the software of DNAMAN and MEGA 4.Results ITS sequences of three samples from the same... |
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| Keywords: | Asparagus cochinchinersis(Lour ) Merr ITS sequences molecular identification genuineness clusteraralysis |
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