COX-2、Ki-67和VEGF在膀胱移行细胞癌中的表达及意义

 目的 探讨环氧化酶-2（COX-2）在膀胱移行细胞癌中的表达及临床意义。方法 应用免疫组织化学法检测50份膀胱移行细胞癌患者手术后存档标本和10份正常膀胱黏膜组织中COX-2、血管内皮生长因子（VEGF）和增生细胞核抗原（Ki-67）的表达。结果 COX-2在膀胱移行细胞癌组织中阳性表达率为80 %，而在正常膀胱黏膜组织中无表达，差异有统计学意义。而且不同分期、不同分级间表达差异亦有统计学意义，即与分级、分期呈正相关；初发组COX-2表达高于复发组；单发组与多发组差异无统计学意义。在膀胱移行细胞癌组织中COX-2的表达与Ki-67的表达呈正相关；COX-2与VEGF在膀胱移行细胞癌组织中的表达呈正相关。结论 COX-2在肿瘤发生、发展中可能起着促进细胞增生的作用。VEGF的表达与COX-2呈正相关，表明VEGF可能是COX-2促进肿瘤血管生成的重要介质

Expression of COX-2,Ki-67 and VEGF in bladder transitional epithelial cancers and their significance

Objective To study COX-2 expression in bladder transitional cell carcinoma in order to explore the relationship between the immunohistochemical staining and biological behavior of bladder cancer. Methods The expression of COX-2 , Ki-67, and VEGF was examined in 50 cases of bladder transitional cell carcinoma and 10 normal bladder mucosa by means of immunohistochemical technique. Results The expression of COX-2 in BTCCs was higher than that in normal bladder mucosa. The positive rate of COX-2 was 80% in BTCCs while it was not detected in normal bladder mucosa. COX-2 was highly positively related to the grade,stage and prognosis of tumors. The expression of COX-2 in poorly differentiated, invasive or re－curring BTCCs was higher than that in well differentiated superficial or nonrecurring ones. But there was not significant difference between the solitary tumour and multiple ones.There were positive correlation both be－tween COX-2 and Ki-67 and between COX-2 and VEGF. Conclusion The high expression of COX-2 may benefit increase in bladder cancer cell proliferative activity. VEGF expression in COX-2 positive tumor was significantly higher than that in COX-2 negative tumor.