姜黄素对脓毒症大鼠血浆ICAM-1水平和预后的影响

目的 探讨姜黄素(curcumine,Cur)治疗脓毒症大鼠的疗效及可能机制。 方法 120只SPF 级SD大鼠随机分为假手术组（Sham组）、盲肠结扎穿孔组（CLP组）、姜黄素治疗组（Cur组），CLP组和Cur组进行盲肠结扎穿孔术，Cur组予姜黄素液2ml/kg （即100mg/kg）腹腔注射，Sham组、CLP组给予2ml/kg生理盐水腹腔注射。各组大鼠于术后0、3、6、12、24、48 小时（h）各时点随机活杀5只动物取血清检测ICAM-1水平，各组剩下10只观察96h内生存时间。动物活杀前进行严重程度评分。 结果 CLP组大鼠术后脓毒症表现最强，Cur组明显减轻，而Sham组没有相关表现或表现轻微。CLP组血浆ICAM-1水平于术后3 h显著升高，至24 h达高峰；而Cur组上升的幅度明显低于CLP组（P﹤0.05），但明显高于Sham组。Cur组生存时间较CLP组明显延长(P<0.01)。结论 姜黄素减轻大鼠脓毒症表现，延长脓毒症大鼠生存时间，改善其预后可能与姜黄素抑制ICAM-1表达有关。

Effect of Curcumine on serum ICAM-1 level and prognosis of septic rats

Objective To investigate the effect of curcumine(Cur) on septic rats and its possible mechanisms. Methods 120 SD rats (specific pathogen free) were randomly divided into sham operation group (Sham group),cecal ligation and puncture (CLP) group (CLP group)and Cur treating group( Cur group). Groups of 5 animals were sacrificed at 0,3,6,12,24 and 48 hours after operation. The remaining animals were observed for survival time after CLP in 96 hours.Blood samples were harvested before animals died. Before sacrifice,the septic severity score were recorded.After sacrifice,the levels of intercellular adhesion molecule-1 (ICAM-1) in plasma were determined. Rusults The degree of severity of sepsis was higher in CLP group than those of other groups; The degree of Cur groups was lower than that of CLP group,but was higher than that of Sham group (both P<0.01). The survival time of septic rats was shorter in CLP group than those in other groups; The survival time in Cur group was shorter than that of Sham group,but was longer than that of CLP group(both P<0.01). The levels of ICAM-1 in plasma rose to the peak at 24 hours after CLP in CLP and Cur groups,but the value in Cur group was significantly lower than that of CLP group at all time points (all P<0.01). Conclusion Curreduces the severity of septic rats and prolongs the survival time, probably associated with inhibition of ICAM-1 expression.