肝细胞癌组织中Ｎｕｍｂ、ｐ５３蛋白的表达及其意义*

目的　观察Ｎｕｍｂ蛋白和ｐ５３蛋白在肝细胞癌（ＨＣＣ）组织中的表达情况及其两者的关系。方法　采用免疫组化ＳＰ法检测３０例ＨＣＣ组织及其癌旁组织、８例肝硬化组织和９例正常肝组织中的Ｎｕｍｂ蛋白和ｐ５３蛋白的表达情况。结果　Ｎｕｍｂ蛋白在ＨＣＣ组织中的阳性表达率为４４.７％，明显低于癌旁组织、肝硬化组织及正常肝组织，差异有统计学意义（Ｐ＜０.０５）；ｐ５３蛋白在ＨＣＣ组织中的阳性表达率为６６.７％，明显高于癌旁组织、肝硬化组织及正常肝组织，差异有统计学意义（Ｐ＜０.０１）；在ＨＣＣ组织中，Ｎｕｍｂ蛋白表达与ｐ５３蛋白表达呈负相关（ｒ＝－０.４６６７，Ｐ＜０.０５）。结论　ＨＣＣ组织中Ｎｕｍｂ蛋白和ｐ５３蛋白表达与非ＨＣＣ组织比较均有变化，两者可能均参与了ＨＣＣ的发生发展过程。

Significance of Numb and p53 proteins' expressions in hepatocellular carcinoma

Objective To discuss significance of Numb and p53 proteins＇ expressions in hepatocellular carcinoma（HCC） and their relationships. Methods The expressions of Numb and p53 were detected by immunohistochemistry in 30 hepatocellular carcinoma tissues and cancer adjacent tissues,8 liver cirrhosis （LC） tissues and 9 normal liver tissues. Results The positive rate of Numb in HCC tissues was significantly lower than that in cancer tissues, LC tissues and normal liver tissues（P 〈 0. 05）. The positive rate of p53 in HCC tissues was significantly higher than that in cancer adjacent tissues, LC tissues and normal liver tissues （P 〈 0. 05 ）. The expression of Numb protein had minus relation to the expression of p53 protein （ r = - 0. 4667,P 〈 0. 05 ）. Conclusion Compared to expressions in non-HCC tissues, expressions of Numb protein and p53 protein in HCC tissues were changed, which might relate to carcinogenesis and development of HCC.