| 一种简单迅速筛选肿瘤基因组中基因突变的方法 |
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| 引用本文： | 于景翠,钟淑琦,傅松滨.一种简单迅速筛选肿瘤基因组中基因突变的方法[J].中国地方病学杂志,2000,19(3):221-223. |
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| 作者姓名： | 于景翠 钟淑琦 傅松滨 |
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| 作者单位： | 哈尔滨医科大学,基础医学院,黑龙江,哈尔滨,10086 |
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| 基金项目： | 黑龙江省科学技术计划项目青年基金 |
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| 摘 要： | 目的 建立一种新的非同位素ＳＳＣＰ分析方法。方法 常规提取人类基因组ＤＮＡ作为模板，ＰＣＲ扩增ｎｍ２３－Ｈ１基因，变性ＰＣＲ产物，６％非变性聚丙烯酰胺凝胶ＳＳＣＰ电泳，银染后分析结果。结果 改进了目前ＰＣＲ－ＳＳＣＰ分析中应用同位素标记的检测方法，采用银染方法，建立起简单迅速的非同位素ＰＣＲ－ＳＳＣＰ分析方法和适宜条件。并将这种方法成功地应用于肿瘤转移抑制基因点突变的检测。结论 非同位素聚合酶链反
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| 关 键 词： | 肿瘤基因组 PCR－SSCP 银染 nm23基因 基因突变 |
Analysis of non-isotopic PCR-SSCP A simple and rapid method for detection of gene mutation in the genomic DNA of human tumors |
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| YU Jing-cui,ZHONG Shu-qi,FU Song-bin.Analysis of non-isotopic PCR-SSCP A simple and rapid method for detection of gene mutation in the genomic DNA of human tumors[J].Chinese Jouranl of Endemiology,2000,19(3):221-223. |
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| Authors: | YU Jing-cui ZHONG Shu-qi FU Song-bin |
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| Abstract: | Objective Establish a simple and rapid method for nonisotopic PCR SSCP.Methods Human genomic DNA was extracted from the samples of human gastric cancers and the controls.And nm23 H1 gene was amplified by polymerase chain reaction.Analysis of single stranded conformation polymorphism was performed on 6% homogenous polyacrylamide gel.Results We have improved and established a method for non isotopic PCR SSCP to detect gene mutations.Conclusions Non isotopic PCR SSCP is a simple and rapid method to detect gene mutations in human tumors. |
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| Keywords: | PCR SSCP Nm23 H1 gene Gene mutation |
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