| 酸枣仁皂苷部位HPLC指纹图谱的研究 |
| |
| 引用本文： | 吴和珍,陈婧,刘焱文. 酸枣仁皂苷部位HPLC指纹图谱的研究[J]. 中药材, 2006, 29(9): 900-903 |
| |
| 作者姓名： | 吴和珍 陈婧 刘焱文 |
| |
| 作者单位： | 湖北中医学院中药资源与中药化学省级重点实验室,湖北,武汉,430061 |
| |
| 基金项目： | 湖北省教育厅资助项目(2002Z0006) |
| |
| 摘 要： | 目的:建立酸枣仁皂苷有效部位HPLC指纹图谱分析方法,研究不同产地酸枣仁药材的质量。方法:采用HPLC-ELSD梯度洗脱的方法进行色谱分离,使用“相似度评价软件”进行数据处理,对不同产地和不同购买地的11批酸枣仁药材皂苷部位进行指纹图谱的研究。结果:确立了酸枣仁药材皂苷部位指纹图谱的高效液相分析条件,不同产地酸枣仁药材皂苷部位指纹图谱有一定差异。结论:采用HPLC-ELSD法制定酸枣仁药材的指纹图谱,其皂苷类成分得到较好分离,方法重现性好,可用于酸枣仁的质量评价。
|
| 关 键 词： | 酸枣仁 HPLC-ELSD 皂苷 指纹图谱 |
| 文章编号： | 1001-4454(2006)09-0900-04 |
| 收稿时间： | 2006-05-10 |
| 修稿时间： | 2006-05-10 |
Studies on HPLC-FPS of the Saponins from Semen Ziziphi Spinosae |
| |
| WU He-zhen,CHEN Jing,LIU Yan-wen. Studies on HPLC-FPS of the Saponins from Semen Ziziphi Spinosae[J]. Journal of Chinese medicinal materials, 2006, 29(9): 900-903 |
| |
| Authors: | WU He-zhen CHEN Jing LIU Yan-wen |
| |
| Affiliation: | Hubei Key Lab of Traditional Chinese Medicine Resources and Chemistry, Hubei College of TCM, Wuhan 430061, China |
| |
| Abstract: | Objective: To establish a method of HPLC-fingerprint spectrum(HPLC-FPS) for the active part in Semen Ziziphi Spinosae(SZS) in order to control the quality of SZS from different places.Methods: The gradient elution mode was applied in chromatographic separation,and data were analysed by"Similarity Evaluation software"to compare the HPLC-FPS of SZS from different places.Results: The conditions for HPLC analysis of SZS were established and the FPS of samples from different habitats showed some differences.Conclusion: All components in the spectrum were separated well and the HPLC fingerprint method is repeatable.The method can be used in quality assessment of SZS. |
| |
| Keywords: | Semen Ziziphi Spinosae HPLC-ELSD Saponins Chromatography fingerprint |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
