| 电子线照射促进大鼠脊髓损伤后运动功能恢复 |
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| 引用本文： | 陈晨,王伟.电子线照射促进大鼠脊髓损伤后运动功能恢复[J].国外医学：物理医学与康复学分册,2009,4(1). |
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| 作者姓名： | 陈晨 王伟 |
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| 作者单位： | 陈晨,CHEN Chen(郑州大学第一附属医院老年病科,郑州,450052);王伟,WANG Wei(华中科技大学同济医学院附属同济医院神经科,武汉,430030)
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| 摘 要： | 目的:观察电子线照射对大鼠脊髓损伤(SCI)后运动功能恢复的影响。方法:36只SD雌性大鼠随机分为假手术组、损伤组和照射组,采用改良Allen’s法制作大鼠脊髓T7～T8损伤模型,照射组在损伤后1 d给予20 Gy电子线照射,损伤组不给予任何干预。采用BBB评分法评定后肢运动功能,免疫荧光组织化学方法观察损伤后4周损伤灶周GFAP表达情况,Western blot对GFAP和生长相关蛋白-43(GAP-43)进行半定量检测。结果:假手术组大鼠运动功能不受影响,损伤组大鼠BBB评分损伤后明显下降,以后逐渐恢复,到4周时达平台期,照射组BBB评分高于损伤组,差异有统计学意义(P<0.05)。免疫组织化学结果显示损伤后4周损伤组的损伤灶周形成胶质瘢痕,照射组未形成明显的胶质瘢痕,GFAP表达较损伤组弱,差异有统计学意义(P<0.05)。Western blot结果显示SCI后4周损伤组和照射组的GFAP表达较假手术组明显增多,照射组较损伤组减低,差异有统计学意义(P<0.05);照射组的GAP-43表达较损伤组增强(P<0.05)。结论:电子线照射可以抑制脊髓损伤后胶质瘢痕的形成,促进运动功能恢复。
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| 关 键 词： | 脊髓损伤 电子线 生长相关蛋白-43 胶质瘢痕 |
Electron Irradiation of Contusion Site Improves Locomotor Recovery in Spinal Cord Injured Rats |
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| CHEN Chen,WANG Wei.Electron Irradiation of Contusion Site Improves Locomotor Recovery in Spinal Cord Injured Rats[J].Neural Injury and Functional Reconstruction,2009,4(1). |
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| Authors: | CHEN Chen WANG Wei |
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| Institution: | CHEN Chen,WANG Wei.Department of Geriatrics,First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China |
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| Abstract: | Objective: To explore the effect of electron irradiation on the locomotor recovery of spinal cord injured rats.Methods: Thirty-six SD female rats were divided randomly into groups sham-operation,spinal cord injury and electron irradiation.The spinal cord injury model was generated according to modified Allen's method.Rats were given electron irradiation after 1 d of spinal cord injury.BBB scale score was measured.Expression level of growth-associated proteins-43(GAP-43) protein was semi-quantified by wester... |
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| Keywords: | spinal cord injury electron irradiation GAP-43 glial scar |
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