大鼠脑出血后细胞黏附分子1 mRNA的表达与血脑屏障通透性的关系

目的探讨大鼠脑出血后细胞间黏附分子1(ICAM-1)mRNA的表达与血脑屏障(BBB)通透性的关系。方法采用自体动脉血注入大鼠尾状核建立脑出血模型,将50只大鼠分成对照组、脑出血组,两组分6小时、1天、3天、5天、7天5个时点,每个时点各5只大鼠。分别采用干湿重法、伊文思兰染色法、逆转录聚合酶链法(RT-PCR)测定不同时间点的脑水含量、血脑屏障通透性(EB含量)和ICAM-1mRNA表达量的变化。结果大鼠出血造模后6小时BBB通透性和ICAM-1表达开始升高,3天达高峰,7天开始下降,出血组和对照组EB含量6小时(0.592±0.106)OD/mg vs(0.309±0.044)OD/mg,3天(0.791±0.520)OD/mg vs(0.315±0.342)OD/mg,7天(0.455±0.082)OD/mg vs(0.308±0.032)OD/mg(均P<0.01)。ICAM-1 6小时1.220±0.044vs 0.884±0.336,3天1.638±0.07vs 0.860±0.057,7天1.282±0.144vs 0.872±0.048(均P<0.01)。BBB通透性与ICAM-1mRNA表达呈正相关(r=0.433,P<0.01),而且与脑水含量变化趋势相一致。结论脑出血后血肿周围组织ICAM-1mRNA表达增高,可能增加血脑屏障通透性,并参与脑水肿形成。

Relationship between cell adhesion molecule-1 mRNA expressibn and blood-brain barrier permeability after experimental cerebral hemorrhage in rats

Objective To investigate the relationship between the mRNA expression of intercellular adhesion molecule-1 （ICAM-1） and blood-brain barrier （BBB） permeability after experimental cerebral hemorrhage in rats. Methods The cerebral hemorrhage models were established by stereotaxie injection of auto-non-anticoagulant artery blood into the caudate nucleus. ICAM-1 expression was detected by RT-PCR,BBB permeability by Evens Blue method and brain water contents by dry-wet mass method. Results In contrast to the control group, ICAM-1 expression increased in cerebral hemorrhage group at 6 hours 1. 220±0. 044, reached peak at 3 days 1. 638 ± 0. 071, then decreased at 5--7 days （ P d0.05） ;BBB permeability increased at 6 hours after cerebral hemorrhage （0. 592±0. 106） OD/mg, reached peak at 3 days （0. 791± 0. 520） OD/mg,and decreased at 7 days gradually. There was significantly positive correlation between ICAM-1 expression and BBB permeability （ r = 0. 433, P 〈0.01）, which was consistent with the change of cerebral edema. Conclusion Cerebral edema may be related with the up-regulation of ICAM-1 expression and the increase of BBB permeability after experimental cerebral hemorrhage.