细胞因子对人单核/巨噬细胞表达CD40和CD40L的影响

目的：探讨细胞因子在γ干扰素（IFN-γ）、肿瘤坏死因子（TNF）和白介素-1（IL-1）对人单核/巨噬细胞表达CD40和CD40L的影响。方法：应用RT-PCR方法半定量测定CD40和CD40LmRNA含量，流式细胞技术检测细胞表面CD40和CD40L表达水平。结果：IFN-γ，TNF和IL-1既增加CD40和CD40LmRNA表达，又能诱导单核/巨噬细胞表面CD40和CD40L高表达，而抗氧化剂VitE能下调细胞因子引起的CD40和CD40L表达，但作用不完全，结论：细胞因子（IFN-γ，TNF和IL-1）能刺激单核/巨噬细胞高表达CD40和CD40L，这种作用可被VitE部分抑制。

Effects of cytokines on the expression of CD40 and CD40 ligand in cultured human monocytes/macrophages

Objective： To investigate the effect of c ytokines (IFN-γ，TNF and IL-1) on the expression of CD40 and CD40 ligand (CD4 0L) in monocytes/macrophages. Methods： The mRNA expression of C D40 and CD40L was measured by RT-PCR and the CD40，CD40L expression on the mono cytes/macrophages were detected by flow cytometric analysis. Results： IFN-γ，TNF and IL-1 could not only significantly up-regulate the mRNA levels of CD40 and CD40L in cultured monocytes/macrophages, but also increase t he expression of CD40 and CD40L. Antioxidant VitE could reduce the expression o f CD40 and CD40L induced by IFN-γ，TNF and IL-1. Conclusion： IFN-γ，TNF and IL-1 can stimulate high expression of CD40 and CD40L . Antio xidant VitE can partially inhibit the expression of CD40 and CD40L induced by cy tokines in cultured monocytes/macrophages.