Effect of streptozotocin on the glutathione S-transferases of mouse liver cytosol |
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| Authors: | C Agius A S Gidari |
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| Institution: | 1. The Second Medical School of Lanzhou University, Lanzhou, Gansu 730000, China;2. Reproductive Medicine Center, Peking University People’s Hospital, Peking 100000, China;3. School of Public Health of Lanzhou University, Lanzhou, Gansu 730000, China;4. Evidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou Gansu 730000, China;5. Chinese GRADE Center, Lanzhou University, Lanzhou, Gansu 730000, China;6. The first Medical School of Lanzhou University, Lanzhou, Gansu 730000, China;7. The Reproductive Medicine Special Hospital of the first hospital of Lanzhou University, Key Laboratory for Reproductive Medicine and Embryo, Lanzhou, Gansu 730000, China;8. Institute of Global Health, University of Geneva, Switzerland;9. Institute of Mathematical Statistics and Actuarial Science, University of Bern, Switzerland;1. School of Economics and Management, China University of Geosciences, Wuhan, 430074, China;2. School of Finance and Economics, Jiangsu University, China;3. Department of Management Sciences, Abbottabad University of Science and Technology, Pakistan;1. Dynamic42 GmbH, Jena, Germany;2. upcyte technologies GmbH, Hamburg, Germany;1. Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street, Rochester, MN 55905, USA;2. Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, 4939 Charles Katz Drive, San Antonio, TX 78229, USA;3. Metabolomics Core, Mayo Clinic, 200 First Street, Rochester, MN 55905, USA;4. Department of Quantitative Health Sciences, Mayo Clinic, 200 First Street, Rochester, MN 55905, USA;5. Department of Laboratory Medicine and Pathology, Division of Anatomic Pathology, Mayo Clinic, 200 First Street, Rochester, MN 55905, USA;6. Departments of Surgery and Immunology, Mayo Clinic, Rochester, MN 55905, USA;7. Department of Medicine, Division of Diabetes, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA;8. Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN 55905, USA |
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| Abstract: | Streptozotocin (STZ) increased the activity of mouse hepatic glutathione (GSH) S-transferases assayed with 1-chloro-2,4-dinitrobenzene. Nicotinamide administered prior to STZ prevented the hyperglycemia indicative of STZ-induced diabetes, but had no effect on the increase in GSH S-transferase activity caused by the drug. Another diabetogenic agent, alloxan, did not alter GSH S-transferase activity. Thus, streptozotocin may be increasing GSH S-transferase activity directly, and not as a result of the diabetic state the drug induces. Two transferases were characterized from mouse liver cytosol. One was a homodimer with a subunit molecular weight of about 28,000 and a pI of about 8.2. The other was also a homodimer with a subunit molecular weight of about 27,500 and a pI of about 9.2. The pI 8.2 GSH S-transferase was induced by STZ, while the pI 9.2 transferase was decreased by the drug. At least one other transferase appeared to be induced by STZ. Two other nitroso compounds, chlorozotocin and diethylnitrosamine, also increased GSH S-transferase activity, suggesting that this effect may be nitroso related. |
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