| 高效液相色谱-示差折光检测法测定补中益气丸中蜂蜜的含量 |
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| 引用本文： | 刘雁鸣,彭飞城,龙海燕,陈得雨.高效液相色谱-示差折光检测法测定补中益气丸中蜂蜜的含量[J].中南药学,2011,9(11):804-807. |
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| 作者姓名： | 刘雁鸣 彭飞城 龙海燕 陈得雨 |
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| 作者单位： | 1. 湖南药用辅料检验检测中心,长沙,410014
2. 湖南省药品检验所,长沙,410001 |
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| 基金项目： | 国家科技部支撑计划子课题 |
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| 摘 要： | 目的建立补中益气丸(蜜丸)蜂蜜中葡萄糖、果糖、蔗糖、麦芽糖含量测定方法,以监控补中益气丸质量。方法 采用高效液相色谱-示差折光检测(HPLC-RD)法;色谱柱为Prevail Carbohydrate ES(250mm×4.6mm,5μm);流动相为乙腈-水(75∶25);流速1.0mL·min-1;柱温:35℃。结果葡萄糖、果糖、蔗糖、麦芽糖对照品分别在17.868～179.14、16.144～161.44、3.016～30.16、6.565～98.475μg线性关系良好,4种成分加样回收率分别为97.1%、101.9%、102.6%、95.9%,RSD分别为0.8%、2.0%、0.8%、1.3%(n=6)。结论该方法操作简便,灵敏度高,重复性好,可用于补中益气丸中蜂蜜的含量测定。
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| 关 键 词： | 补中益气丸 高效液相色谱法 蜂蜜 葡萄糖 果糖 蔗糖 麦芽糖 |
HPLC-RD determination of honey in Buzhong Yiqi Wan |
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| LIU Yan-ming,PENG Fei-cheng,LONG Hai-yan,CHEN De-yu.HPLC-RD determination of honey in Buzhong Yiqi Wan[J].Central South Pharmacy,2011,9(11):804-807. |
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| Authors: | LIU Yan-ming PENG Fei-cheng LONG Hai-yan CHEN De-yu |
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| Institution: | LIU Yan-ming1,PENG Fei-cheng2*,LONG Hai-yan1,CHEN De-yu1(1.Hunan Inspection and Testing Center of Pharmaceutical Excipients,Changsha 410014,2.Hunan Institute for Drug Contral,Changsha 410001) |
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| Abstract: | Objective To evaluate the quality of Buzhong Yiqi Wan （honey pills） to determine the contents of glucose, fructose, sucrose and maltose. Methods An HPLORD system was adopted with a Prevail Carbohydrate ES （250 mm ×4.6 mm, 5 μm）. The mobile phase was acetonitrite-water （75 ： 25）, the flow was 1.0 mL · min^-1 , and the column temperature was 35 ℃. Results The standard curves of glucose, fructose, sucrose and maltose showed good linearity over 17. 868-179.14, 16. 144-161.44, 3. 016-30.16, and 6. 565-98. 475μg, respectively. The average recovery was 97. 1%, 101.9%,102.6%,and 95.9%, with RSD=0. 8%, 2.0%, 0.8%and 1.3%（n=6）, respectively. Conclusion The method is simple, sensitive, reproducible, and effective for the determination of honey in Buzhong Yiqi Wan （honey pills）. |
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| Keywords: | Buzhong Yiqi Wan HPLC honey glucose fructose sucrose maltose |
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