| 酒石酸亚铁比色法测定黄鹂芽中茶多酚的含量 |
| |
| 引用本文： | 刘蓓蓓,陈胜璜,邹菊英,李琴雯,陈汉骏. 酒石酸亚铁比色法测定黄鹂芽中茶多酚的含量[J]. 中南药学, 2011, 9(10): 740-741. DOI: 10.3969/j.issn.1672-2981.2011.10.006 |
| |
| 作者姓名： | 刘蓓蓓 陈胜璜 邹菊英 李琴雯 陈汉骏 |
| |
| 作者单位： | 1. 湖南中医药大学药学院,长沙,410208
2. 湖南中医药大学药学院,长沙410208;湖南中医药大学中药现代化重点实验室,长沙410208 |
| |
| 基金项目： | 湖南省研究生科研创新基金项目(编号:CX2009B176) |
| |
| 摘 要： | 目的测定黄鹂芽茶中茶多酚的含量。方法采用国家标准酒石酸亚铁比色法,在535nm处测定吸光度,用对照品标准曲线法测定黄鹂芽茶中茶多酚的含量。结果茶多酚在10～100μg.L-1呈良好的线性关系;回归方程:Y=8.104 X-0.000 9,r=0.999 8,平均加样回收率为96.5%,RSD为2.1%(n=5)。结论酒石酸亚铁比色法测定茶多酚准确、可靠,可作为黄鹂芽中茶多酚的含量测定方法。
|
| 关 键 词： | 黄鹂芽 茶多酚 酒石酸亚铁比色法 含量测定 |
Determination of tea polyphenol in Huangli buds by ferrous tartrate method |
| |
| LIU Bei-bei,CHEN Sheng-huang,ZOU Ju-ying,LI Qin-wen,CHEN Han-jun. Determination of tea polyphenol in Huangli buds by ferrous tartrate method[J]. Central South Pharmacy, 2011, 9(10): 740-741. DOI: 10.3969/j.issn.1672-2981.2011.10.006 |
| |
| Authors: | LIU Bei-bei CHEN Sheng-huang ZOU Ju-ying LI Qin-wen CHEN Han-jun |
| |
| Affiliation: | LIU Bei-bei1,CHEN Sheng-huang1,2,ZOU Ju-ying1,LI Qin-wen1,CHEN Han-jun1(1.School of Pharmacy,Hunan University of Traditional Chinese Medicine,Changsha 410208,2.Key Lab of Modernization of Chinese Medicine,Changsha 410208) |
| |
| Abstract: | Objective To determine tea polyphenol（TP） in leaves of Pistacia chinensis Bunge.Methods Ferrous tartaric acid colorimetry was used to determine the content of TP at 535 nm according to the standard curve.Results Tea polyphenol had good liner relationship at 10-100 μg·L-1.The regression equation was as follows：Y=8.104X-0.000 9（r=0.999 8）,the recovery was 96.5%,and RSD=2.1%（n=5）.Conclusion The ferrous tartaric acid colorimetry method is sensitive and accurate,which can be used for the determination of tea polyphenol in leaves of Pistacia chinensis Bunge. |
| |
| Keywords: | Huangli bud tea polyphenol ferrous tartrate method content determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
