反相高效液相色谱法检测羊水和胎盘中的乳酸依沙吖啶

目的应用反相高效液相色谱法(RP-HPLC)测定羊水和胎盘中的乳酸依沙吖啶。方法 采用甲醇浸泡溶解,7%三氯乙酸沉淀蛋白的方法,提取样品后进行高效液相色谱测定。色谱柱:用Acclaim C18色谱柱(250mm×4.6mm,5μm);流动相:甲醇-乙腈-水(50∶28∶22);紫外检测器:检测波长:272nm;柱温:35℃;流速:0.8mL·min-1。结果乳酸依沙吖啶在1.0～10.0μg·mL-1与峰面积呈现良好线性关系(r=0.999 9),回收率为95.5%～99.5%,日内RSD为1.2%～1.7%,日间RSD为1.7%～2.2%。结论该方法简便、快速、灵敏、准确,可作为临床检测方法。

RP-HPLC assay of ethacridine lactate in the amniotic fluid and placenta

Objective To establish an HPLC method to the monitor ethacridine lactate in the placenta and amniotic flu id. Methods The sample was dissolved in the methanol and 7% trichloroacetic acid （TDA） was used to subside the protein. The column was acclaim C18 （250 mm×4.6 mm, 5 μm）, the mobile phase was methanol-acetonitrile-water （50 ： 28 ： 22）, the wavelength was 272 nm, the column temperature was 35 ℃, and the flow rate was 0. 8 mL · min^-1. Results At 1.0-10.0 μg · mL^-1, a good linearity （r=0. 999 9） was shown. The recovery was 95.5%- 99.5%, and intra-dayRSD was 1.2%-1.7%, and inter-day RSD was 1.7%-2.2%. Conclusion The method is simple, rapid, sensitive, and can be used in clinical tests.