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Expression of G protein estrogen receptor (GPER) on membrane of mouse oocytes during maturation |
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| Authors: | Yi-Ran Li Chun-E Ren Quan Zhang Ji-Chun Li Ri-Cheng Chian |
| Affiliation: | 1. Department of Obstetrics and Gynecology, Ningxia Medical University, Yinchuan, China 2. Department of Obstetrics and Gynecology, McGill University, Montreal, Canada 3. Department of Obstetrics and Gynecology, Weifang Medical Colleage, Weifang, China 4. Center for Reproductive Medicine, Women and Infants Hospital of Qingdao, Qingdao, China 5. State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China |
| Abstract: | PurposeTo determine expression of G-protein estrogen receptor (GPER) in mouse oocyte membrane during maturation.MethodsThe expression of GPER from different maturation stages of oocytes, in vivo and in vitro matured oocytes as well as aging oocytes was examined by immune-fluorescence GPR30 antibody and the images were analyzed by laser scanning confocal microscope. Further confirmation was performed by Western blots for cell fractionation.ResultsSignificant fluorescent signal was observed on the surface of mouse oocytes. The image expression was lower in germinal vesicle (GV) stage than mature metaphase-II (M-II) stage oocytes. There was high expression in in-vivo matured oocytes compared to in vitro matured oocytes. The highest expression was observed in aging oocytes compared with other oocytes.ConclusionsThe changes of expression of GPER on mouse oocytes plasma membrane confirm oocyte membrane maturation, suggesting that those changes of GPER may be related to the functional role of oocyte maturation. |
| Keywords: | Mouse Oocyte Membrane Maturation GPER |
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