| 血红素氧合酶1和血红素氧合酶2在雌性去势大鼠阴道平滑肌中的表达 |
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| 引用本文： | 夏川,姜睿.血红素氧合酶1和血红素氧合酶2在雌性去势大鼠阴道平滑肌中的表达[J].中华男科学杂志,2009,15(2):110-114. |
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| 作者姓名： | 夏川 姜睿 |
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| 作者单位： | 1. 峨眉山市人民医院泌尿外科
2. 泸州医学院附属医院泌尿外科,四川,泸州,646000 |
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| 摘 要： | 目的:研究血红素氧合酶1(HO-1)、血红素氧合酶2(HO-2)在雌性去势大鼠阴道平滑肌中的表达,探讨雌激素对阴道平滑肌HO-1、HO-2表达的影响。方法:雌性8周龄SD大鼠随机分为A(去卵巢2周组)、B(去卵巢4周组)、C(假手术2周组)、D(假手术4周组)4组,每组6只,放免法测定各组雌性大鼠血清雌二醇水平,运用免疫组化和RT-PCR对HO-1、HO-2在阴道平滑肌进行定位和半定量分析。结果:术后A与C、B与D组血清雌二醇比较有显著差异(P<0.05);HO-1、HO-2免疫组化定位在阴道平滑肌细胞胞质内,A组表达显著低于C组;阴道平滑肌组织中HO-1、HO-2mRNA表达在A、B组均显著低于C、D组,阴道中HO-1mRNA、HO-2mRNA下降与血清雌二醇下降相关。结论:HO-1、HO-2在雌性大鼠阴道平滑肌中有表达,低水平的雌激素与HO-1、HO-2表达降低有关,雌激素可能经HO/CO途径在阴道平滑肌中发挥生理作用。
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| 关 键 词： | 性功能障碍 血红素氧合酶1 血红素氧合酶2 免疫组化 女性 |
Expressions of HO-1 and HO-2 in the Vaginal Smooth Muscle of Ovariectomized Female Rats |
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| XIA Chuan,JIANG Rui.Expressions of HO-1 and HO-2 in the Vaginal Smooth Muscle of Ovariectomized Female Rats[J].National Journal of Andrology,2009,15(2):110-114. |
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| Authors: | XIA Chuan JIANG Rui |
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| Institution: | XIA Chuan,JIANG RuiDepartment of Urology,The Affiliated Hospital of Luzhou Medical College,Luzhou,Sichuan 646000,China |
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| Abstract: | Objective:To analyze the changes of heme oxygenase-1(HO-1)and heme oxygenase-2(HO-2)in the vaginal smooth muscle of ovariectomized female rats and to investigate the effects of estrogen on the expressions of HO-1 and HO-2 in the vaginal smooth muscle.Methods:Twenty-four 8-week old female SD rats were equally randomized into Group A(2-week castrated),B(4-week castrated),C(2-week sham operation)and D(4-week sham operation).The levels of plasma estradiol were measured by radioimmunoassay for all the rats and t... |
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| Keywords: | sexual dysfunction heme oxygenase-1 heme oxygenase-2 immunohistochemistry female |
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