| 加味佛手散抑制大鼠子宫内膜异位症血管生成及其机制 |
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| 引用本文： | 王小翠,杨洋,陈刚,邓礼娟,徐晓玉. 加味佛手散抑制大鼠子宫内膜异位症血管生成及其机制[J]. 中国药理学通报, 2011, 27(3): 350-354 |
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| 作者姓名： | 王小翠 杨洋 陈刚 邓礼娟 徐晓玉 |
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| 作者单位： | 1. 西南大学药学院,重庆市药效评价工程技术研究中心,重庆,400716
2. 重庆工商大学药物化学与化学生物学研究中心,重庆,400067 |
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| 基金项目： | 科技部"重大新药创制"专项，西南大学引进人才启动基金 |
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| 摘 要： | 目的探讨复方中药新药加味佛手散抑制子宫内膜异位症(EMS)血管生成作用及机制。方法采用子宫内膜自体移植法建立大鼠EMS模型,免疫组化和Western blot方法检测异位内膜组织中微血管密度(MVD)、血管内皮生长因子(VEGF)和内皮抑素(endostatin)蛋白的表达,放射免疫分析法(RIA)分别检测大鼠血清和异位内膜组织中前列腺素E2(PGE2)的含量。结果加味佛手散0.045、0.09、0.18 g.kg-1能有效抑制异位内膜组织体积的增生,加味佛手散0.09、0.18 g.kg-1能降低MVD,降低VEGF蛋白的表达,降低大鼠血清和异位内膜组织中PGE2的含量,加味佛手散0.18 g.kg-1能够升高endostatin蛋白的表达。结论加味佛手散具有抗血管生成作用,其作用机制与抑制VEGF、促进endostatin蛋白表达有关。
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| 关 键 词： | 加味佛手散 子宫内膜异位症 血管生成 大鼠 VEGF endostatin |
Inhibitory effects of Jiawei Foshousan on angiogenesis of endometriosis in rats model and its mechanism |
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| WANG Xiao-cui,YANG Yang,CHEN Gang,DENG Li-juan,XU Xiao-yu. Inhibitory effects of Jiawei Foshousan on angiogenesis of endometriosis in rats model and its mechanism[J]. Chinese Pharmacological Bulletin, 2011, 27(3): 350-354 |
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| Authors: | WANG Xiao-cui YANG Yang CHEN Gang DENG Li-juan XU Xiao-yu |
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| Affiliation: | WANG Xiao-cui1,YANG Yang1,CHEN Gang2,DENG Li-juan1,XU Xiao-yu1 (1.College of Pharmaceutical Sciences,Southwest University,Chongqing Engineering Research Center for Pharmacodynamics Evaluation,Chongqing 400716,China,2.Research Center of Medical Chemistry and Chemical Biology,Chongqing Technology and Business University,Chongqing 400067,China) |
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| Abstract: | Aim To explore the effect and the mechanism of Jiawei Foshousan on angiogenesis of endometriosis(EMS)in rats.Methods The rat model of EMS was established by surgical implant of the endometrial tissue which belong to its body.We explored microvessels density(MVD)by the immunohistochemical test as well as the expression of vascular endothelial growth factor(VEGF)protein and endostatin protein in ectopic endometriotic tissue by the immunohistochemical test and western blot.The level of secretion of prostagland... |
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| Keywords: | VEGF endostatin |
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