黄芩总黄酮对博莱霉素致大鼠肺纤维化的干预作用

目的探讨黄芩总黄酮(total flavonoids of scutellariabaicalensis georgi,TFSB)对博莱霉素致大鼠肺纤维化的干预作用。方法气管内注入博莱霉素制备大鼠肺纤维化模型,给予TFSB灌服28 d,每天1次后,计算肺系数、测定肺组织和血清中肺纤维化部分相关生化指标,观察大鼠肺泡炎和肺纤维化程度,RT-PCR检测转化生长因子-β1(TGF-β1)、Smad2、Smad3 mRNA的表达水平,Western blot检测平滑肌肌动蛋白α(α-SMA)与过氧化物酶体增殖因子活化受体γ(PPAR-γ)的蛋白表达水平。结果与模型组相比,TFSB各剂量组大鼠肺组织羟脯氨酸(HYP)含量、血清与肺组织匀浆中丙二醛(MDA)含量降低(P<0.05,P<0.01),血清与肺组织匀浆中谷胱甘肽(GSH)含量、血清超氧化物歧化酶(SOD)活性、血清总抗氧化能力(T-AOC)升高(P<0.05,P<0.01),TFSB各剂量组大鼠肺泡炎和肺纤维化程度降低(P<0.01),RT-PCR和Western blot结果显示:TGFβ-1、Smad2、Smad3 mRNA的表达水平升高(P<0.05),α-SMA蛋白表达水平降低(P<0.05),PPAR-γ蛋白表达水平升高(P<0.05)。结论 TFSB对博莱霉素致大鼠肺纤维化有一定的防治作用,其机制可能与机体内的抗氧化和下调TGF-β1信号通路有关。

Effects of total flavonoids of scutellaria baicalensis georgi(TFSB)on bleomycin-induced pulmonary fibrosis in rats

Aim To study the effects of total flavonoids of scutellaria baicalensis georgi(TFSB)on bleomycin-induced pulmonary fibrosis in rats and investigate its mechanisms.Methods The pulmonary fibrosis model was estalished by intratracheal injection of Bleomycin.After 28 d,rats were treated with TFSB by intragastric administration,and the pathomorphology,the biochemistrical index in lung tissue and blood were measured and analyzed.The effects of TFSB on the expressions of transforming growth factor-β1(TGF-β1),Smad2/3 mRNA and the expressions of alpha-smooth muscle actin(α-SMA)and peroxisome proliferator-activated receptor-gamma(PPAR-γ)protein in lung tissue of rats were assayed by RT-PCR and Western blot.Results Compared with the model group,the contents of MDA in serum and lung tissue and the contents of HYP were decreased(P<0.05,P<0.01),the contents of GSH,T-AOC and SOD in serum were increased(P<0.05,P<0.01),the contents of GSH in lung tissue were increased(P<0.01).TFSB could obviously reduce alveolitis and pulmonary fibrosis(P<0.05,P<0.01).The expressions of TGF-β1 and Smad2/3 mRNA in lung tissue of rats were decreased(P<0.05),and the expression of α-SMA in lung tissue of rats was decreased(P<0.05).In contrast,the expression of PPAR-γ protein in lung tissue of rats were increased(P<0.05).Conclusion TFSB has therapeutic effects of bleomycin-induced pulmonary fibrosis in rats,which might be related with the anti-oxidation and the inhibition of TGF-β1 signaling pathway.