海参岩藻聚糖硫酸酯对小鼠急性酒精中毒防治作用的研究

目的研究海参岩藻聚糖硫酸酯(fucoidan isolatedfrom sea cucumber,SC-FUC)对小鼠急性酒精中毒的防治作用。方法将♂ICR小鼠随机分为5组:正常对照组、模型对照组、益肝灵阳性对照组、SC-FUC低、高剂量组(50和100mg.kg-1.d-1),连续灌胃SC-FUC 4 d,于末次给药后1 h按体重灌胃乙醇体积分数为56%的白酒(15 ml.kg-1),分别记录醉酒和醒酒时间,检测血清丙氨酸氨基转移酶(ala-nine aminotransferase,ALT)和天门冬氨酸氨基转移酶(aspar-tate aminotransferase,AST)活性,肝脏中乙醇脱氢酶(alcoholdehydrogenase,ADH)、乙醛脱氢酶(acetaldehyde dehydrogen-ase,ALD)、谷胱甘肽过氧化酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)活性,以及丙二醛(malondialdehyde,MDA)含量。结果 SC-FUC能有效推迟小鼠的醉酒时间,缩短醒酒时间,明显降低小鼠血清ALT和AST活力(P<0.01),提高肝脏ADH、ALD和抗氧化酶GSH-Px和CAT活力,降低MDA含量,对SOD活力无明显影响。结论 SC-FUC能明显促进乙醇在小鼠肝脏中的代谢速度,提高肝脏的抗氧化能力,具有较好的防治醉酒和保护肝脏作用。

Study on the preventive and therapeutic effects of fucoidan isolated from sea cucumber on acute alcoholism in mice

Aim To study the preventive and therapeutic effects of fucoidan isolated from sea cucumber(SC-FUC) on acute alcoholism in mice.Methods Male ICR mice were randomly divided into five groups: normal control group,model control group,Yiganling positive control group,SC-FUC low and high dose groups(50 and 100 mg·kg-1·d-1).The mice were treated daily by ig of SC-FUC,Yiganling or normal saline for 4 days.One hour after the last ig,the mice were given liquor(15 ml·kg-1).The time of drunkenness and sobriety were recorded.At the end of the experiment,the activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum were tested and the effect of SC-FUC on the activities of alcohol dehydrogenase(ADH),acetaldehyde dehydrogenase(ALD),antioxidase and the content of malondialdehyde(MDA) in liver were observed.Results SC-FUC could put off the time of drunkenness and shorten the time of sobriety of mice.It could reduce the activities of ALT and AST in serum(P<0.01) and improve the activities of ADH,ALD and antioxidase in liver.The content of MDA was significantly decreased.Conclusion SC-FUC has good effects on drunkenness prevention and liver protection through the way of enhancing the metabolism of ethanol and the antioxidation ability in mice liver.