UPLC-MS/MS同时测定Beagle犬血浆中原儿茶酸、异荭草素和野黄芩苷

目的建立Beagle犬血浆中同时测定原儿茶酸、异荭草素和野黄芩苷的超高效液相色谱-串联质谱分析方法。方法 Beagle犬单次股静脉注射88 mg.kg-1的注射用复方荭草(冻干粉针),分时取血处理。色谱采用Waters BEH C18反相柱,流动相为乙腈-水(含0.1%甲酸)梯度洗脱,质谱采用多反应离子监测(MRM)方式进行检测,用于定量分析的离子对分别为原儿茶酸m/z109.0→m/z152.8、异荭草素m/z449.2→m/z299.1、野黄芩苷m/z463.1→m/z287.1。结果原儿茶酸、异荭草素和野黄芩苷的线性范围分别为0.002～0.569、0.022～5.450和0.024～5.860 mg·L-1,方法的准确度,日内、日间精密度和稳定性均符合要求。结论该方法快速、灵敏、专属性强,可用于原儿茶酸、异荭草素和野黄芩苷的药代动力学研究。

Simultaneous determination of protocatechuic acid, isoorientin and scutellarin in Beagle dog plasma by UPLC-MS/MS

Aim To develop an UPLC-MS method for simultaneous determination of protocatechuic acid,isoorientin and scutellarin in Beagle dog plasma.Methods Blood samples from Beagle dogs were collected at scheduled time points after intravenous injection of 88 mg·kg-1 Compound Hongcao injection.The analysis column was a Waters BEH C18 column,the mobile phase consisted of acetonitrile and water(0.1% formic acid included),and the detection was carried out in the multiple reaction monitoring(MRM) mode.The ion pairs for quantitation were m/z109.0→m/z152.8 for protocatechuic acid,m/z449.2→m/z299.1 for isoorientin and m/z463.1→m/z287.1 for scutellarin,respectively.Results The linear range of protocatechuic acid,isoorientin and scutellarin was 0.002~0.569,0.022~5.450,and 0.024~5.860 mg·L-1,respectively.The accuracy,intra-and inter-day precisions,and the stability of the analytes met the requirements.Conclusion The above mentioned method is sensitive,specific,and suitable for pharmacokinetic studies of protocatechuic acid,isoorientin and scutellarin.