卡介苗联合Poly I:C接种对新生期小鼠脾脏T细胞的影响

目的 研究卡介苗(BCG)联合Poly I:C新生期接种对小鼠脾脏T细胞功能亚群发育的影响.方法 新生清洁级BALB/c小鼠32只,分4组:对照组、BCG组、Poly I:C组、BCG和Poly I:C联合接种组,新生期接种4周后取脾细胞用流式细胞仪检测CD3+ CD8+ IFN-γ+、CD3+ CD8- IFN-γ+、CD3+ CD8+ IL-4+ 、CD3+ CD8- IL-4+、CIM+ Foxp3+ T细胞的比例,其分别代表Tc1、TH1、Tc2、TH2和调节性T细胞(Treg)亚群.结果 BCG组、Poly I:C组、BCG和Poly I:C联合接种组TH1、Tc1细胞比例明显高于对照组(P＜0.05或P＜0.01),3个接种组间比较差异无统计学意义;Tc2、TH2和Treg比例各组间比较差异没有统计学意义;3个接种组TH1/TH2和总IFN-γ/IL-4比值明显高于对照组(P＜0.05或P＜0.01),联合接种组TH1/TH2比值高于BCG组(P＜0.05),Tc1/Tc2比值各组间差异没有统计学意义;CD4+ Foxp3+ T细胞比例各组间比较差异没有统计学意义.结论 新生期BCG和Poly I:C接种均能促进TH1、Tc1细胞亚群的发育,提高TH1/TH2比值,其联合接种在TH1/TH2水平可能具有一定的协同作用,但不能影响CD4+ Foxp3+ Treg细胞的比例.

The co-vaccination effects of BCG and Poly I:C on the development of T cell subsets in neonatal BALB/c mice

Objective To explore the effects of BCG and Poly I:C co-vaccination on the development of spleen T cell subsets of neonatal BALB/c mice. Methods Neonatal BALB/c mice were inoculated with BCG and/or Poly I:C intraperitoneally within 2-3 d after birth. Four weeks later, spleen cells of mice were isolated and the percentage of CD3+ CD8+ IFN-γ+,CD3+ CD8-IFN-γ+,CD3+ CD8+ IL-4+,CD3+ CD8- IL-4+,CD4+ Foxp3+ T cells,which represent Tc1,TH1,Tc2,TH2,Treg cells,respectively,were tested by flow cytometry at single cell level,and the ratios of TH 1/TH 2 and Tc1/Tc2 were calculated. Results The percentages of TH1 and Tc1 cells of BCG-vaccinated mice,Poly I:C-vaccinated mice and BCG plus Poly I:C-vaccinated mice were significantly higher than that of control mice(P＜0.05 or P＜0.01),and there was no difference among the three vaccinated group. The ratios of TH1/TH2 and total IFN-γ/IL-4 of the three vaccinated groups were higher than that of control group,but not the ratio of Tc1/Tc2. The TH1/TH2 ratio of BCG plus Poly I:C-vaccinated group was higher than that of BCG-vaccinated group(P＜0.05).The percentages of Trge cells showed no difference among the four groups(P＞0.05). Conclusion BCG and Poly I:C co-vaccination can significantly increase the number of Tc1 and TH 1 cells and TH 1/TH2 ratio in spleen cells. BCG and Poly I:C vaccination may have a synergistic effect on TH 1/TH2 ratio of spleen cells in neonataI mice. The percentage of CD4+ Foxp3+ T cells among four groups showed no significant difference.