脂质体介导的p53基因转染对鼻咽癌细胞作用的研究

目的 通过转染野生型p53基因,观察其对鼻咽癌细胞CNE2生长的影响.方法 将克隆有野生型p53基因的pUHD10-3-p53质粒,用脂质体(lipofectamine)介导的方法转染人鼻咽癌CNE2细胞,以RT-PCR检测p53基因的表达,通过细胞生长曲线、AO/EB染色和流式细胞术等方法对转染细胞的生物学行为进行检测.结果 人鼻咽癌CNE2细胞转染p53基因后,基因在细胞中表达增加,细胞的生长受到抑制,在荧光显微镜下观察到凋亡细胞增多,流式细胞技术显示细胞凋亡率增高.结论 采用脂质体转染野生型p53基因的方法,可抑制人鼻咽癌CNE2细胞的生长,诱导鼻咽癌细胞凋亡.

The effect of the growth of nasopharyngeal carcinoma transfecting with lipofectamine-mediated p53 gene

Objective To investigate the effect of wild type p53(wt-p53)gene on the growth and apoptosis of nasopharyngeal carcinoma by transfected it into nasopuaryngeal carcinoma CNE2 cell lines,effect.Methods The nasopharyngeal carcinoma CNE2 cell lines was transfected with plasmid pUHD10-3-p53 recombined with lipofectamine-mediateed wt-p53 gene.3-(4,5-Dimethy-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromid(MTT)technique was used to analyse the growth of nasopharyngeal carcinoma CNE2 cell lines and apoptosis of cells was evaluated by AO/EB dyeing and flow cytometry.The expression of p53 was detected by RT-PCR method.Results After transfected with wt-p53 gene,the growth of nasopharyngeal carcinoma CNE2 cell lines was obviously inhibited.Apoptosis cells became more and the apotosis rate of CNE2 cells was higher.The high level of p53 expression was observed in pUHD10-3-p53 transfection cells.Conclusion The growth of the nasopharyngeal carcinoma CNE2 cell lines were obviously inhibited by transfecting with plasmid pUHD10-3-p53 recombined with lipofectamine-mediated wt-p53 gene.