| 脂肪组织源性干细胞分步诱导分化为神经元样细胞 |
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| 引用本文： | 陈盼盼,张丽华,董为人,刘杰明,张易,乔伟光,陈英华,赵姝,郭家松.脂肪组织源性干细胞分步诱导分化为神经元样细胞[J].南方医科大学学报,2011,31(3):512-517. |
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| 作者姓名： | 陈盼盼 张丽华 董为人 刘杰明 张易 乔伟光 陈英华 赵姝 郭家松 |
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| 作者单位： | 陈盼盼,董为人,张易,乔伟光,陈英华,赵姝,郭家松,CHEN Pan-pan,DONG Wei-ren,ZHANG Yi,QIAO Wei-guang,CHEN Ying-hua,ZHAO Shu,GUO Jia-Song(南方医科大学基础医学院组织学与胚胎学教研室,广东,广州,510515);张丽华,ZHANG Li-hua(佛山市科学技术学院基础医学院,广东,佛山,528305);刘杰明,LIU lie-ming(南方医科大学附属顺德区人民医院神经外科,广东,顺德,528300) |
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| 基金项目： | 广东省高校优秀中青年人才基金，佛山市科技攻关项目，佛山市卫生局医学科研立项 |
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| 摘 要： | 目的探讨脂肪组织源性干细胞(ADSCs)诱导分化为神经元样细胞的可行性。方法 ADSCs分离自雄性SD大鼠附睾脂肪垫,差速贴壁法纯化ADSCs,传至第3代培养细胞行流式细胞术行表型鉴定(CD106,CD11b,CD45,CD90,CD29,CD49d),并进行成脂诱导。取鉴定后的ADSCs用分步诱导法,即先用含10 ng/ml碱性成纤维细胞生长因子(bFGF)、20ng/ml表皮生长因子(EGF)、2%B27的DMEM/F12培养基I培养,诱导其向神经干细胞转分化,再用含10 ng/ml胶质细胞源性神经营养因子(GDNF),10 ng/ml脑源性神经营养因子(BDNF),1μmol/L维甲酸(RA)的培养基II诱导分化为神经元样细胞(NLCs),免疫细胞化学法鉴定细胞表面标记物巢蛋白(nestin),神经细胞特异性标志微管相关蛋白(MAP2),神经元核蛋白(NeuN)。结果分离纯化的ADSCs CD90、CD29表达强阳性;成脂诱导分化后细胞油红O染色阳性;ADSCs经培养基I培养7 d后聚集成球样细胞团悬浮于培养液中,表达nestin;继而用培养基II诱导分化4 h后细胞球逐渐贴壁,球周边少数细胞向外发出突起,形似神经元,MAP2、NeuN均呈阳性。结论特定条件下,ADSCs在体外可被逐步诱导分化为NLCs。
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| 关 键 词： | 脂肪组织源性干细胞 分化 神经干细胞 神经元 |
A two-step protocol for induction of rat adipose tissue-derived stem cells into neuron-like cells |
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| CHEN Pan-pan,ZHANG Li-hua,DONG Wei-ren,LIU lie-ming,ZHANG Yi,QIAO Wei-guang,CHEN Ying-hua,ZHAO Shu,GUO Jia-Song.A two-step protocol for induction of rat adipose tissue-derived stem cells into neuron-like cells[J].Journal of Southern Medical University,2011,31(3):512-517. |
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| Authors: | CHEN Pan-pan ZHANG Li-hua DONG Wei-ren LIU lie-ming ZHANG Yi QIAO Wei-guang CHEN Ying-hua ZHAO Shu GUO Jia-Song |
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| Institution: | CHEN Pan-pan1,ZHANG Li-hua2,DONG Wei-ren1,LIU Jie-ming3,ZHANG Yi1,QIAO Wei-guang1,CHEN Ying-hua1,ZHAO Shu1,GUO Jia-Song1 1Department of Embryology and Histology,School of Basic Medical Sciences,Southern Medical University,Guangzhou 510515,China,2School of Medicine,Foshan University,Foshan 528000,3Department of Neurosurgery,Shunde People's Hospital,Shunde 528300 |
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| Abstract: | Objective To investigate the differentiation potential of rat adipose tissue-derived cells(ADSCs) into neuron-like cells in vitro using a two-step induction protocol.Methods ADSCs isolated from the epididymal fat pads in male SD rats by means of differential attachment were cultured in vitro and subjected to adipogenic induction.After flow cytometric identification of the cell surface antigens CD106,CD11b,CD45,CD49d,CD90 and CD29,the third-passage ADSCs were induced to transdifferentiate into neural stem ce... |
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| Keywords: | adipose tissue-derived stem cells differentiation neural stem cells neurons |
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